鸡核糖体DNA：基因频率及通过R环杂交进行纯化

Chicken ribosomal DNA: gene frequency and purification by R-loop hybridization.

作者信息

Lehmann C, Warnhoff M, Knochel W, Lange D, Born J, Tiedemann H

出版信息

Mol Biol Rep. 1979 Feb 15;4(4):217-21. doi: 10.1007/BF00777557.

Abstract

The genes for ribosomal RNA of chicken are present in about 200--240 copies per haploid genome. The ribosomal genes were separated from the bulk of DNA by different methods. In CsCl-actinomycin D gradients the GC-rich ribosomal DNA is shifted to the lower density side of the bulk of DNA. After hybridization with iodinated ribosomal RNA under conditions where R-loops are formed ribosomal DNA has an increased density. The R-loops can be separated from the bulk of DNA in Cs2-SO4 gradients.

摘要

鸡核糖体RNA的基因在单倍体基因组中约有200 - 240个拷贝。核糖体基因通过不同方法与大部分DNA分离。在CsCl -放线菌素D梯度中，富含GC的核糖体DNA向大部分DNA的低密度侧移动。在形成R环的条件下与碘化核糖体RNA杂交后，核糖体DNA的密度增加。R环可在Cs2 - SO4梯度中与大部分DNA分离。