Shandil R K, Vinayak V K
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandidarh, India.
Med Microbiol Immunol. 1990;179(5):263-9. doi: 10.1007/BF00192464.
Immune sera from 15 patients with cured amoebic liver abscess were used to recognise the antigens of Entamoeba histolytica (HMI) by immunoblotting. The amoebic proteins most frequently recognised by sera from patients with cured amoebic liver abscess had molecular masses of 8, 13, 18, 22, 29, 38, 45, 67 and 94 kDa. Six plasma membrane-associated amoebic proteins of molecular mass 29, 38, 45-67 complex, 85 and 94 kDa were strongly recognised by such sera. Two plasma membrane-associated antigens of 108 and 129 kDa were not recognised by any sera. None of the crude or plasma membrane-associated antigens were recognised by sera from five patients of idiopathic ulcerative colitis, five patients of persistent giardiasis and five normal healthy subjects. Identification of such antigens, especially plasma membrane-associated antigens may pave a way to develop specific diagnostic and immunoprotective agents.
使用15例已治愈阿米巴肝脓肿患者的免疫血清，通过免疫印迹法识别溶组织内阿米巴（HMI）的抗原。已治愈阿米巴肝脓肿患者血清最常识别的阿米巴蛋白分子量为8、13、18、22、29、38、45、67和94 kDa。血清强烈识别6种分子量分别为29、38、45 - 67复合体、85和94 kDa的与质膜相关的阿米巴蛋白。两种分子量为108和129 kDa的与质膜相关的抗原未被任何血清识别。5例特发性溃疡性结肠炎患者、5例持续性贾第虫病患者和5名正常健康受试者的血清未识别任何粗抗原或与质膜相关的抗原。识别此类抗原，尤其是与质膜相关的抗原，可能为开发特异性诊断和免疫保护剂铺平道路。
