Veterinary Public Health, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Vet J. 2011 Sep;189(3):306-11. doi: 10.1016/j.tvjl.2010.07.017. Epub 2010 Sep 6.
Forty-six Salmonella enterica subspecies enterica serovar Enteritidis (S. Enteritidis) strains were isolated from chicken meat, faeces, and eggshells collected from hatcheries throughout Korea. The strains were examined for the presence of antimicrobial resistance and virulence genes. All 46 isolates were resistant to at least one of 21 antibiotics used in this study, 30 (65.2%) were resistant to three or more antimicrobials, and a single remarkable isolate was resistant to 15 antimicrobials. The isolates were primarily resistant to penicillins, sulfisoxazole, streptomycin, tetracycline and quinolones. The high rate of resistance in S. Enteritidis strains, sometimes to multiple drugs, may complicate future options for treating human infections. Nineteen of the 21 penicillin resistant isolates carried the bla(TEM) gene, while one strain, resistant both to penicillins and ceftriaxone, carried the bla(CTX-M) gene. Thirty-seven of the 45 sulfisoxazole resistant isolates carried sul2, and 23/24 streptomycin resistant isolates carried both strA and strB. All 10 tetracycline resistant isolates carried the tet(A) gene. Most isolates harboured both SPI-1 and SPI-2-associated genes, and the spv operon, which are known to be associated with human infections. The presence of these genes suggests that these strains could give rise to public health problems if dispersed in the general human population.
从韩国各地孵化场采集的鸡肉、粪便和蛋壳中分离出了 46 株肠炎沙门氏菌亚种肠炎血清型（S. Enteritidis）。这些菌株被检测是否存在抗药性和毒力基因。所有 46 株分离株均对本研究中使用的 21 种抗生素中的至少一种具有抗药性，30 株（65.2%）对三种或更多种抗生素具有抗药性，而单个显著分离株对 15 种抗生素具有抗药性。这些分离株主要对青霉素、磺胺异噁唑、链霉素、四环素和喹诺酮类药物具有抗药性。肠炎沙门氏菌菌株的抗药性率很高，有时对多种药物具有抗药性，这可能会使未来治疗人类感染的选择复杂化。在 21 株青霉素耐药分离株中，有 19 株携带 bla(TEM)基因，而一株同时对青霉素和头孢曲松具有耐药性的菌株携带 bla(CTX-M)基因。在 45 株磺胺异噁唑耐药分离株中，有 37 株携带 sul2，23/24 株链霉素耐药分离株携带 strA 和 strB。所有 10 株四环素耐药分离株均携带 tet(A)基因。大多数分离株均携带 SPI-1 和 SPI-2 相关基因以及 spv 操纵子，这些基因与人类感染有关。这些基因的存在表明，如果这些菌株在普通人群中传播，可能会引发公共卫生问题。
