Department of Bacteriology, Mycology and Immunology, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Microbiol Immunol. 2012 Apr;56(4):254-61. doi: 10.1111/j.1348-0421.2012.00429.x.
To date, no information has been available on the molecular bases of antimicrobial resistance in Salmonella spp. from poultry in Egypt or even in Africa. Therefore, the objective of this study was to analyze, at the molecular level, the mechanisms of multidrug-resistance in isolates of Salmonella recovered from diseased broilers in Egypt. Twenty-one Salmonella isolates were identified; 13 of these isolates were Salmonella enterica serovar Enteritidis and eight Salmonella enterica serovar Typhimurium. 17 (81%). Salmonella isolates displayed multidrug resistance phenotypes, particularly against ampicillin, streptomycin, spectinomycin, kanamycin, tetracycline, chloramphenicol, and trimethoprim/sulfamethoxazole. PCR and DNA sequencing identified class 1 integrons in nine (42.9%) isolates and class 2 integrons in three (14.3%) isolates. The identified resistance genes within class 1 integrons were aminoglycoside adenyltransferase type A, aadA1, aadA2 and aadA5 and dihydrofolate reductase type A, dfrA1, dfrA5, dfrA12, dfrA15 and dfrA17. The β-lactamase encoding genes bla(TEM-1) and bla(CMY-2) and florfenicol resistance gene floR were also identified. Furthermore, the tetracycline resistance gene tet(A) was identified in 14 (66.7%) Salmonella isolates. To the best of our knowledge, this is the first report of the molecular basis of antimicrobial resistance in Salmonella spp. isolated from poultry in Africa.
迄今为止，关于埃及乃至非洲家禽源沙门氏菌的抗菌药物耐药性的分子基础尚无任何信息。因此，本研究的目的是在分子水平上分析从埃及病鸡分离的沙门氏菌的多药耐药机制。共鉴定出 21 株沙门氏菌分离株；其中 13 株为肠炎沙门氏菌血清型，8 株为鼠伤寒沙门氏菌血清型。17 株（81%）沙门氏菌分离株表现出多药耐药表型，特别是对氨苄西林、链霉素、壮观霉素、卡那霉素、四环素、氯霉素和甲氧苄啶/磺胺甲噁唑耐药。PCR 和 DNA 测序在 9 株（42.9%）分离株中鉴定出 1 类整合子，在 3 株（14.3%）分离株中鉴定出 2 类整合子。1 类整合子中鉴定出的耐药基因包括氨基糖苷类腺苷转移酶类型 A、aadA1、aadA2 和 aadA5 以及二氢叶酸还原酶类型 A、dfrA1、dfrA5、dfrA12、dfrA15 和 dfrA17。还鉴定出β-内酰胺酶编码基因 bla(TEM-1)和 bla(CMY-2)以及氟苯尼考耐药基因 floR。此外，在 14 株（66.7%）沙门氏菌分离株中鉴定出四环素耐药基因 tet(A)。据我们所知，这是首次报道非洲家禽源沙门氏菌的抗菌药物耐药性的分子基础。
