Vladyko A S, Kunitskaia L Ia, Kras'ko A G, Bystrova S I, Pyzhova N S, Lukashevich I S
Vopr Virusol. 1990 Nov-Dec;35(6):488-92.
Eighteen hybrid lines secreting recombinant monoclonal antibodies to Lassa virus were produced by fusion of mouse splenocytes with antibody-secreting X-63 myeloma cells. Interrelations between the structure and reactivity of the antibodies were studied by different serological and immunochemical methods. Monoclonal antibodies were divided into different groups according to their serological properties and macromolecular structure. A comparative analysis of the structure and reactivity of the recombinant monoclonal antibodies showed that the light and heavy Ig-specific chains could form the reactive antibodies when the chains were present in different paratopes of Ig molecules.
通过将小鼠脾细胞与分泌抗体的X-63骨髓瘤细胞融合，产生了18株分泌抗拉沙病毒重组单克隆抗体的杂交瘤细胞系。采用不同的血清学和免疫化学方法研究了这些抗体的结构与反应性之间的相互关系。根据单克隆抗体的血清学特性和大分子结构将其分为不同的组。对重组单克隆抗体的结构和反应性进行的比较分析表明，当轻链和重链存在于Ig分子的不同互补决定区时，它们可以形成有反应活性的抗体。
