Department of Aquaculture, Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
Res Vet Sci. 2011 Aug;91(1):103-109. doi: 10.1016/j.rvsc.2010.08.009. Epub 2010 Sep 9.
A feeding trial was conducted for 60 days to study the immunomodulatory role of three different immunostimulants yeast extract (YE), brewer's yeast (BY) and spirulina (SP) in Labeo rohita fingerlings. Four hundred and fifty fingerlings (avg. wt 3.35±0.15 g) were randomly distributed in ten treatments and fed with either of ten iso-nitrogenous and iso-caloric semi-purified diets, prepared with three incremental levels (1%, 2% and 4%) of different immunostimulants except the control. Growth parameters did not vary significantly (p>0.05) among the experimental groups. Haematology and serum parameters was performed before Aeromonas hydrophila challenge whereas respiratory burst activity was analysed following challenge. The respiratory burst activity, total leucocyte count, serum total protein and globulin was significantly higher (p<0.05) in YE 1% supplemented group. The survival (%) after challenging with A. hydrophila was also highest in the YE fed groups. The results indicate that among the different sources and levels of immunostimulants, YE at lower inclusion level is more effective in promoting the immune status of L. rohita fingerlings.
进行了为期 60 天的饲养试验，研究了三种不同免疫增强剂酵母提取物（YE）、啤酒酵母（BY）和螺旋藻（SP）在罗非鱼幼鱼中的免疫调节作用。将 450 尾幼鱼（平均体重 3.35±0.15 g）随机分配到 10 个处理组中，用 10 种等氮和等热量的半纯化饲料喂养，除对照组外，饲料中添加了三种递增水平（1%、2%和 4%）的不同免疫增强剂。实验组之间的生长参数没有显著差异（p>0.05）。在受到嗜水气单胞菌挑战前进行血液学和血清参数分析，而在挑战后分析呼吸爆发活性。YE 1%添加组的呼吸爆发活性、总白细胞计数、血清总蛋白和球蛋白显著升高（p<0.05）。在受到嗜水气单胞菌感染后，YE 喂养组的存活率（%）也最高。结果表明，在不同来源和水平的免疫增强剂中，较低添加水平的 YE 更能有效促进罗非鱼幼鱼的免疫状态。
