Department of Microbiology and Molecular Genetics and Center for Infectious Disease Research, Medical College of Wisconsin, Milwaukee, WI, USA.
Virology. 2010 Nov 25;407(2):185-95. doi: 10.1016/j.virol.2010.08.012. Epub 2010 Sep 9.
All viruses with a positive-stranded RNA genome replicate their genomic RNA in association with membranes from the host cell. Here we demonstrate a novel organelle source of replication membranes for human rhinovirus 1A (HRV-1A). HRV-1A infection induces fragmentation of the Golgi apparatus, and Golgi membranes are rearranged into vesicles of approximately 250-500 nm diameter. The newly distributed Golgi membranes co-localize with viral RNA replication templates, strongly suggesting that the observed vesicles are the sites of viral RNA replication. Expression of the HRV-1A 3A protein induces alterations in the Golgi staining pattern similar to those seen during viral infection, and expressed 3A localizes to the Golgi-derived membranes. Taken together, these data show that in HRV-1A infection, the 3A protein plays a role in fragmenting the Golgi complex and generating vesicles that are used as the site of viral RNA replication.
所有正链 RNA 基因组的病毒都与宿主细胞膜结合来复制其基因组 RNA。在这里，我们展示了人鼻病毒 1A（HRV-1A）复制膜的一种新型细胞器来源。HRV-1A 感染诱导高尔基体的碎片化，高尔基体膜被重组成大约 250-500nm 直径的囊泡。新分布的高尔基体膜与病毒 RNA 复制模板共定位，强烈表明观察到的囊泡是病毒 RNA 复制的部位。HRV-1A 的 3A 蛋白的表达诱导高尔基体染色模式的改变，类似于在病毒感染期间观察到的改变，并且表达的 3A 定位到高尔基体衍生的膜上。总之，这些数据表明在 HRV-1A 感染中，3A 蛋白在分裂高尔基体复合物和产生用作病毒 RNA 复制部位的囊泡方面发挥作用。
