Repiso Ada, Saavedra Pedro, Casal José, Lawrence Peter A
Department of Zoology, Downing Street, Cambridge, UK.
Development. 2010 Oct;137(20):3411-5. doi: 10.1242/dev.047126. Epub 2010 Sep 8.
The larval ventral belts of Drosophila consist of six to seven rows of denticles that are oriented, some pointing forwards, some backwards. We present evidence that denticle orientation is determined almost entirely by Dachsous and Fat, one of two planar cell polarity systems. If we change the distribution of Dachsous we can alter the polarity of denticles. We suggest that the orientation of the individual denticle rows, in both the anterior compartment (which mostly point backwards) and the posterior compartment (which point forwards), is determined by the opposing slopes of a Dachsous/Fat gradient. We show, by altering the concentration gradients of Dachsous during development, that we can change the polarity of the denticles made by larval cells as they progress between the first and third larval instars without mitosis.
果蝇幼虫的腹带由六到七排小齿组成，这些小齿的方向各不相同，有些向前，有些向后。我们提供的证据表明，小齿的方向几乎完全由平面细胞极性系统之一的达索蛋白（Dachsous）和脂肪蛋白（Fat）决定。如果我们改变达索蛋白的分布，就可以改变小齿的极性。我们认为，在前部区域（大多数小齿向后）和后部区域（小齿向前）中，各个小齿排的方向是由达索蛋白/脂肪蛋白梯度的相反斜率决定的。我们通过在发育过程中改变达索蛋白的浓度梯度表明，我们可以在幼虫细胞在第一和第三龄幼虫之间无有丝分裂地发育过程中，改变它们所形成的小齿的极性。
