Department of Physical Medicine and Rehabilitation, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
Dent Mater J. 2010 Oct;29(5):518-28. doi: 10.4012/dmj.2009-138. Epub 2010 Sep 4.
The aim of this study was to analyze the effects of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on fibrous tissue formation and cell adhesion plaque (CAP)-forming reactions. Silastic elastomer (SE) plates coated (experimental group) and uncoated (control group) with MPC polymer were prepared for in vivo and in vitro experiments. For the in vivo animal experiments, SE plates were implanted subcutaneously in the rat dorsal region. At 4, 8, and 12 weeks, thicknesses of the fibrous tissue capsules in the experimental group were lower than in the control group. Likewise, the amount of collagen in the experimental group was lower than that of the control group. For the in vitro cell culture experiments, KMST-6 fibroblast cells in the experimental group demonstrated enhanced cell migration, accompanied with a weaker expression of vinculin and a larger amount of filopodia. Furthermore, weaker expressions of paxillin, talin, and ROCK1, but stronger expression of cofilin, were observed in the experimental group. Taken together, these results suggested that MPC polymer regulated fibrous tissue formation by modulating cell adhesion through changes in local CAPs and downstream signaling.
本研究旨在分析 2-甲基丙烯酰氧乙基磷酸胆碱（MPC）聚合物对纤维组织形成和细胞黏附斑（CAP）形成反应的影响。制备了涂覆（实验组）和未涂覆（对照组）MPC 聚合物的硅橡胶弹性体（SE）板，用于体内和体外实验。在体内动物实验中，SE 板被皮下植入大鼠背部。在第 4、8 和 12 周，实验组的纤维组织囊厚度低于对照组。同样，实验组的胶原蛋白含量也低于对照组。在体外细胞培养实验中，实验组的 KMST-6 成纤维细胞表现出增强的细胞迁移，伴随着 vinculin 表达减弱和更多的丝状伪足。此外，实验组中 paxillin、talin 和 ROCK1 的表达减弱，而 cofilin 的表达增强。综上所述，这些结果表明，MPC 聚合物通过改变局部 CAP 和下游信号来调节细胞黏附，从而调节纤维组织形成。
