Base for Drug Clinical Trial, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, PR China.
Fitoterapia. 2011 Mar;82(2):184-9. doi: 10.1016/j.fitote.2010.08.019. Epub 2010 Sep 7.
The protective effect of berberine against antioxidant, antilipid peroxidation in serum and liver tissue, and positive transcription elongation factor b (P-TEFb) expression in liver tissue of type 2 diabetic rats was investigated. Overnight fasted rats were intraperitoneally injected 35 mg/kg streptozotocin. Diabetic rats were admitted after 2 weeks and given a high-carbohydrate/high-fat diet to induce hyperlipidemias. From week 16, diabetic rats were treated with 75, 150, 300 mg/kg berberine, 100mg/kg fenofibrate or 4 mg/kg rosiglitazone for another 16 weeks. P-TEFb (composed of cyclin-dependent kinase 9 and cyclin T1) mRNA and protein expression in liver tissue were detected by real time PCR and immunohistochemistry, respectively. Berberine significantly up-regulated the declined cyclin-dependent kinase 9, cyclin T1 mRNA and protein expression in diabetic rat liver. Berberine obviously decreased malondialdehyde level and increased catalase, superoxide dismutase, glutathione peroxidase, and glutathione activities in liver tissue and serum of diabetic rats. These results suggest that the effects of berberine on up-regulation of P-TEFb expression, antioxidant and antilipid peroxidation may be related to its protective potential on diabetes.
研究了小檗碱对 2 型糖尿病大鼠血清和肝组织抗氧化、抗脂质过氧化和肝组织阳性转录伸长因子 b（P-TEFb）表达的保护作用。 overnight fasted rats 是 overnight-fasted rats 的缩写，overnight-fasted rats 意为“禁食过夜的大鼠”，即大鼠在实验前一晚禁食。 streptozotocin 是链脲佐菌素的缩写，是一种常用的诱导糖尿病的化学物质。 diabetic rats 是糖尿病大鼠的缩写。 high-carbohydrate/high-fat diet 是高碳水化合物/高脂肪饮食的缩写，这种饮食通常用于诱导肥胖和代谢综合征。 hyperlipidemias 是高脂血症的缩写，指血液中脂质（如胆固醇和甘油三酯）水平升高。 cyclin-dependent kinase 9 和 cyclin T1 是细胞周期蛋白依赖性激酶 9 和细胞周期蛋白 T1 的缩写，它们在细胞周期的 G1 期到 S 期转换中发挥重要作用。 real time PCR 是实时聚合酶链反应的缩写，是一种用于定量检测特定 DNA 或 RNA 序列的技术。 immunohistochemistry 是免疫组织化学的缩写，是一种用于检测和定位组织或细胞中的特定蛋白质的技术。 malondialdehyde 是丙二醛的缩写，是脂质过氧化的一种产物，可作为氧化应激的标志物。 catalase 是过氧化氢酶的缩写，是一种抗氧化酶，可分解过氧化氢为水和氧气。 superoxide dismutase 是超氧化物歧化酶的缩写，也是一种抗氧化酶，可将超氧自由基转化为过氧化氢和氧气。 glutathione peroxidase 是谷胱甘肽过氧化物酶的缩写，可将过氧化物转化为无害的物质。 glutathione 是谷胱甘肽的缩写，是一种重要的抗氧化剂和细胞内还原剂。 protective potential 是保护潜力的缩写。
