Clinical application of FTIR imaging: new reasons for hope.

In the 1990s, Fourier transform infrared (FTIR) imaging arrived as an analytical tool for the biological sciences. However, major limitations have appeared with respect to modern techniques of clinical imaging; slow acquisition of data, diffraction limitations, inability to image living biosystems, and weak sensitivity of detectors. Recent technological developments have demonstrated that FTIR imaging can be used to image living biosamples at the surface of specific crystals, lateral resolution can reach 100 nm without diffraction limits, and real-time imaging is accessible. These analytical improvements, in conjunction with industrial efforts in providing a new generation of high photon flux IR sources and more sensitive detectors, will give FTIR imaging a 'second chance' to be introduced into the clinic.