Newman A P, Ferro-Novick S
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
Bioessays. 1990 Oct;12(10):485-91. doi: 10.1002/bies.950121006.
Several complementary approaches have been fruitful in the study of transport from the ER to the Golgi complex in yeast. Mutational analysis has led to the identification of genes required for this process, many of which are now being studied at the molecular and biochemical level. In the case of SEC18, DNA sequence analysis has demonstrated homology to a factor needed for transport in mammalian in vitro systems. In addition, the events that take place at this stage of the secretory pathway have been reconstituted in vitro.
在酵母中，几种互补方法在研究从内质网到高尔基体复合体的转运过程中取得了丰硕成果。突变分析已导致鉴定出该过程所需的基因，其中许多基因目前正在分子和生化水平上进行研究。就SEC18而言，DNA序列分析已证明其与哺乳动物体外系统转运所需的一种因子具有同源性。此外，分泌途径这一阶段发生的事件已在体外得到重建。
