Department of Otorhinolaryngology, Head and Neck Surgery, Ernst Moritz Arndt University Greifswald, Greifswald, Germany.
Skin Pharmacol Physiol. 2010;23 Suppl:35-40. doi: 10.1159/000319602. Epub 2010 Sep 8.
In animal wound models, accelerated wound closure has been shown by use of polihexanide applied in antimicrobially effective concentrations. Additionally, an increased ATP production of keratinocytes in vitro induced by polihexanide was demonstrated and interpreted as a stimulatory effect on cell proliferation. Based on these results and the clinical reports on improved wound healing after introduction of polihexanide for preoperative antisepsis in the nasal cavity, polihexanide was tested in a wound model on respiratory ciliary epithelial cells allowing measurement of the healing process after artificial injury. 0.5 μg/ml polihexanide accelerated wound healing in terms of proliferation and migration significantly after an exposure time of 1 and 96 h. At a concentration of 1 μg/ml polihexanide, the stimulation of wound healing was significantly increased only after an exposure time of 96 h. This is the first study to demonstrate acceleration of wound healing in a standardized in vitro model using an epithelial cell line. Considering the present results and previous reports on the impact of polihexanide on wound healing, the conclusion is drawn that the positive effect of polihexanide on wound healing is a separate, dose-dependent effect independent of its antiseptic properties.
在动物创伤模型中，聚己双胍以具有抗菌效果的浓度使用时，已显示出加速创伤闭合的作用。此外，聚己双胍在体外诱导角质形成细胞中 ATP 产生增加，并被解释为对细胞增殖的刺激作用。基于这些结果以及关于在鼻腔术前消毒中引入聚己双胍后改善伤口愈合的临床报告，聚己双胍在呼吸纤毛上皮细胞的创伤模型中进行了测试，允许测量人工损伤后的愈合过程。在暴露时间为 1 和 96 小时后，0.5μg/ml 的聚己双胍在增殖和迁移方面显著加速了伤口愈合。在 1μg/ml 的聚己双胍浓度下，仅在暴露时间为 96 小时后，伤口愈合的刺激作用才显著增加。这是第一项使用上皮细胞系在标准化体外模型中证明伤口愈合加速的研究。考虑到目前的结果和以前关于聚己双胍对伤口愈合影响的报告，得出的结论是，聚己双胍对伤口愈合的积极影响是一种独立的、剂量依赖性的效应，与其抗菌特性无关。
