Wiegand Cornelia, Abel Martin, Ruth Peter, Hipler Uta-Christina
Department of Dermatology, University Medical Center Jena, Jena, Germany.
Wound Repair Regen. 2009 Sep-Oct;17(5):730-8. doi: 10.1111/j.1524-475X.2009.00536.x.
Wound healing is compromised by critical colonization and infection with bacteria. Hence, antimicrobial agents are used clinically to decrease the bacterial load and promote wound healing. Polihexanide (PHMB) has been found to be effective against a broad spectrum of micro-organisms and is increasingly utilized in rinsing solutions or in combination with wound dressings because of its good biocompatibility. In the present study, a co-culture of human keratinocytes and Staphylococcus aureus was established to serve as an in vitro model for infected wounds. Incubation of keratinocytes with increasing concentrations of S. aureus led to a dose-dependent decline of cell viability and proliferation. Lactate dehydrogenase release and interleukin-8 liberation were found to be elevated under these conditions. Polihexanide dose-dependently was able to protect keratinocytes from bacterial damage and re-establish normal human cell proliferation in vitro. Furthermore, a dressing consisting of biocellulose derived from Acetobacter xylinum with the addition of polihexanide was adept to safeguard keratinocytes against S. aureus. In conclusion, the co-culture system presented embodies a valuable tool as a model system for infected cells in a non-healing wound. Furthermore, the results obtained support the favorable function of polihexanide in the treatment of infected chronic wounds.
伤口愈合会因细菌的严重定植和感染而受到损害。因此，抗菌剂在临床上用于降低细菌载量并促进伤口愈合。聚己缩胍（PHMB）已被发现对多种微生物有效，并且由于其良好的生物相容性，越来越多地用于冲洗液或与伤口敷料联合使用。在本研究中，建立了人角质形成细胞和金黄色葡萄球菌的共培养体系，作为感染伤口的体外模型。用浓度不断增加的金黄色葡萄球菌孵育人角质形成细胞导致细胞活力和增殖呈剂量依赖性下降。在这些条件下，发现乳酸脱氢酶释放和白细胞介素-8释放增加。聚己缩胍能够剂量依赖性地保护角质形成细胞免受细菌损伤，并在体外重新建立正常的人类细胞增殖。此外，一种由木醋杆菌衍生的生物纤维素添加聚己缩胍组成的敷料能够保护角质形成细胞免受金黄色葡萄球菌的侵害。总之，所呈现的共培养体系作为非愈合伤口中感染细胞的模型系统是一种有价值的工具。此外，所获得的结果支持聚己缩胍在治疗感染性慢性伤口中的良好作用。
