BRG1 有助于 RNA 聚合酶 II 在体内延伸过程中克服核小体障碍。
BRG1 helps RNA polymerase II to overcome a nucleosomal barrier during elongation, in vivo.
机构信息
Centro Andaluz de Biología Molecular y Medicina Regenerativa Consejo Superior de Investigaciones Científicas, Avenida Americo Vespucio, 41092 Seville, Spain.
出版信息
EMBO Rep. 2010 Oct;11(10):751-7. doi: 10.1038/embor.2010.131. Epub 2010 Sep 10.
Abstract
RNA polymerase II (RNAPII) transcribes genes in a chromatin context. We have designed a system to investigate the role of chromatin remodelling during elongation in vivo, which involves inserting a strong nucleosome-positioning sequence between a promoter and a reporter gene. Our data indicate that a nucleosome positioned in the body of a transcription unit impairs RNAPII progression, provokes RNAPII accumulation upstream to the positioned nucleosome and reduces transcription. By using this system, we show that BRG1, the enzymatic motor of the SWI-SNF chromatin-remodelling complex, is recruited to the positioned nucleosome in a transcription elongation-dependent manner and facilitates traversal of the nucleosome by RNAPII.
摘要
RNA 聚合酶 II（RNAPII）在染色质环境中转录基因。我们设计了一个系统来研究在体内延伸过程中染色质重塑的作用，该系统涉及在启动子和报告基因之间插入一个强核小体定位序列。我们的数据表明，位于转录单元体部的核小体阻碍了 RNAPII 的延伸，导致 RNAPII 在定位核小体的上游积累，并减少转录。通过使用该系统，我们表明 BRG1，SWI-SNF 染色质重塑复合物的酶促马达，以转录延伸依赖性的方式被招募到定位核小体，并促进 RNAPII 穿越核小体。
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