Thounaojam Menaka C, Jadeja Ravirajsinh N, Dandekar Deven S, Devkar Ranjitsinh V, Ramachandran A V
Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The M.S. University of Baroda, Vadodara 390002, Gujarat, India.
Exp Toxicol Pathol. 2012 Mar;64(3):217-24. doi: 10.1016/j.etp.2010.08.009. Epub 2010 Sep 15.
The present study was aim to evaluate protective role of Sida rhomboidea.Roxb (SR) extract against high fat diet/fatty acid induced pathophysiological alterations in experimental model of non-alcoholic steatohepatitis (NASH). Effect of SR extract on plasma levels of markers of hepatic damage, plasma and hepatic lipids, mitochondrial oxidative stress, status of enzymatic and non-enzymatic antioxidants and histopathological changes in liver tissue were evaluated in high fat diet fed C57BL/6J mice. Also, the effect of SR supplementation on lipid accumulation, lipid peroxidation, cytotoxicity and cell viability were evaluated in oleic acid treated HepG2 cells. Supplementation of NASH mice with SR extract prevented high fat diet induced elevation in plasma marker enzymes of liver damage, plasma and hepatic lipids, mitochondrial oxidative stress and compromised enzymatic and non-enzymatic antioxidant status. Further, addition of SR extract to in vitro HepG2 cells minimized oleic acid induced lipid accumulation, higher lipid peroxidation, cytotoxicity and reduced cell viability. These in vivo and in vitro studies suggest that SR extract has the potential of preventing high fat/fatty acid induced NASH mainly due to its hypolipidemic and antioxidant activities.
本研究旨在评估菱叶梵天花提取物对高脂饮食/脂肪酸诱导的非酒精性脂肪性肝炎（NASH）实验模型病理生理改变的保护作用。在高脂饮食喂养的C57BL/6J小鼠中，评估了菱叶梵天花提取物对肝损伤标志物血浆水平、血浆和肝脏脂质、线粒体氧化应激、酶促和非酶促抗氧化剂状态以及肝组织病理变化的影响。此外，在油酸处理的HepG2细胞中评估了补充菱叶梵天花提取物对脂质积累、脂质过氧化、细胞毒性和细胞活力的影响。给NASH小鼠补充菱叶梵天花提取物可预防高脂饮食诱导的肝损伤血浆标志物酶、血浆和肝脏脂质、线粒体氧化应激以及酶促和非酶促抗氧化剂状态受损的升高。此外，在体外HepG2细胞中添加菱叶梵天花提取物可使油酸诱导的脂质积累、更高的脂质过氧化、细胞毒性最小化，并降低细胞活力。这些体内和体外研究表明，菱叶梵天花提取物具有预防高脂/脂肪酸诱导的NASH的潜力，主要是由于其降血脂和抗氧化活性。
