Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The MS University of Baroda, Vadodara, Gujarat, India.
Food Chem Toxicol. 2010 Dec;48(12):3424-31. doi: 10.1016/j.fct.2010.09.015. Epub 2010 Sep 16.
This study evaluates the protective role of Clerodendron glandulosum.Coleb (CG) aqueous extract against high fat diet/fatty acid induced lipotoxicity in experimental models of non-alcoholic steatohepatitis (NASH). Supplementation of NASH mice with CG extract (1% and 3% in high fat diet for 16 weeks) prevented high fat diet induced elevation in liver enzymes, plasma and hepatic lipids, mitochondrial oxidative stress and compromised enzymatic and non-enzymatic antioxidant status and histopathological damage to hepatocytes. Furthermore, results from in vitro study indicates, addition of CG extract (20-200 μg/ml for 24h) to HepG2 cells minimizes oleic acid induced lipid accumulation, higher lipid peroxidation, cytotoxicity and reduced cell viability. These in vivo and in vitro studies suggest that CG extract has the potential of preventing high fat/fatty acid induced NASH.
本研究评估了罗勒腺萼木（Clerodendron glandulosum.Coleb，CG）水提物对非酒精性脂肪性肝炎（NASH）实验模型中高脂肪饮食/脂肪酸诱导的脂毒性的保护作用。在 NASH 小鼠中补充 CG 提取物（在高脂肪饮食中添加 1%和 3%，持续 16 周）可防止高脂肪饮食引起的肝酶、血浆和肝脂质升高、线粒体氧化应激以及酶和非酶抗氧化状态受损和肝细胞的组织病理学损伤。此外，体外研究的结果表明，向 HepG2 细胞中添加 CG 提取物（24 小时内 20-200μg/ml）可最大程度地减少油酸诱导的脂质积累、更高的脂质过氧化、细胞毒性和降低细胞活力。这些体内和体外研究表明，CG 提取物具有预防高脂肪/脂肪酸诱导的 NASH 的潜力。
