在检测泌尿生殖系统衣原体感染中，对Pharmacia衣原体酶免疫分析、免疫诊断酶免疫分析及细胞培养法的比较。

Comparison of Pharmacia Chlamydia EIA, IDEIA and cell culture in the detection of urogenital chlamydial infection.

作者信息

Bygdeman S M, Lidbrink P, Nahkiaisoja M, Parkhede U

机构信息

Karolinska Institute, Department of Clinical Bacteriology, Huddinge Hospital, Stockholm, Sweden.

出版信息

Int J STD AIDS. 1990 May;1(3):199-204. doi: 10.1177/095646249000100310.

DOI:10.1177/095646249000100310

PMID:2083294

Abstract

Two commercial test kits, Pharmacia Chlamydia EIA (PhEIA) and IDEIA Chlamydia Test, for the identification of Chlamydia trachomasis and McCoy cell culture were compared in urogenital specimens. The sediments of the transportation buffers of specimens with discordant results were investigated for elementary bodies (EB) with fluorescein-labelled antichlamydial antibodies. The prevalence of chlamydial infection among the men was 16% (48 of 293), 47 culture positive and one EB positive, and among the women 10% (10 of 97), 10 culture positive. In men, the sensitivity of PhEIA, IDEIA and culture was 71%, 40% and 98%, respectively. In women, irrespective of site, corresponding figures were 100%, 80% and 100%. The specificity and positive predictive values were 100% for both enzyme immunoassays in men and women. The low sensitivity of IDEIA could not be explained by the degree of infection as measured by the number of inclusion bodies in cell culture, the presence of antigen as measured by the number of EBs or the sampling order.

摘要

对两种用于鉴定沙眼衣原体的商业检测试剂盒——Pharmacia衣原体酶免疫分析（PhEIA）和IDEIA衣原体检测，以及 McCoy 细胞培养法，在泌尿生殖系统标本中进行了比较。对结果不一致的标本运输缓冲液沉淀物，用荧光素标记的抗衣原体抗体检测原体（EB）。男性衣原体感染率为16%（293例中的48例），47例培养阳性，1例EB阳性；女性感染率为10%（97例中的10例），10例培养阳性。在男性中，PhEIA、IDEIA和细胞培养法的敏感性分别为71%、40%和98%。在女性中，无论感染部位如何，相应数字分别为100%、80%和100%。两种酶免疫分析在男性和女性中的特异性和阳性预测值均为100%。IDEIA敏感性低的原因不能用细胞培养中包涵体数量所衡量的感染程度、EB数量所衡量的抗原存在情况或采样顺序来解释。