Department of Chemistry and Biochemistry, National Chung Cheng University, Chiayi 62102, Taiwan.
Biosens Bioelectron. 2010 Nov 15;26(3):1036-42. doi: 10.1016/j.bios.2010.08.047. Epub 2010 Aug 20.
A facile and label-free biosensing method has been developed for determining an osteoarthritis concerned cytokine, interleukin-1β (IL-1β), in synovial fluids. The biosensing technique, fiber-optic particle plasmon resonance (FOPPR), is based on gold nanoparticles-modified optical fiber where the gold nanoparticle surface has been modified by a mixed self-assembled monolayer for further conjugation of anti-IL-1β antibody and minimization of nonspecific adsorption. Upon binding of IL-1β to anti-IL-1β on the gold nanoparticle surface, the absorbance of the gold nanoparticle layer on the optical fiber changes and the signal change is enhanced through multiple total internal reflections along the optical fiber. Results show that the detection of IL-1β in synovial fluid by this sensor agrees quantitatively with the clinically accepted enzyme-linked immunosorbent assay (ELISA) method but a much shorter analysis time is required (<10 min). The sensor response versus log concentration of IL-1β was linear (r=0.9947) over the concentration range of 0.050-10 ng/mL and a limit of detection (LOD) of 21 pg/mL (1.2 pM) was achieved. Such a LOD for IL-1β (17 kDa) represents a major advancement in the field of real-time monitoring of low molecular weight proteins in complex biological fluids.
已经开发出一种用于检测与骨关节炎相关细胞因子白细胞介素 1β（IL-1β）的简便且无需标记的生物传感方法，用于检测滑液中的 IL-1β。生物传感技术，光纤颗粒等离子体共振（FOPPR），基于金纳米粒子修饰的光纤，其中金纳米粒子表面已通过混合自组装单分子层进行修饰，以进一步连接抗 IL-1β抗体并最小化非特异性吸附。当 IL-1β与金纳米粒子表面上的抗 IL-1β结合时，光纤上的金纳米粒子层的吸光度会发生变化，并且通过光纤内的多次全内反射来增强信号变化。结果表明，该传感器对滑液中 IL-1β的检测与临床认可的酶联免疫吸附测定（ELISA）方法在定量上一致，但所需的分析时间更短（<10 分钟）。传感器的响应与 IL-1β的对数浓度呈线性关系（r=0.9947），在 0.050-10ng/mL 的浓度范围内，检测限（LOD）为 21pg/mL（1.2pM）。这种 IL-1β（17kDa）的 LOD 代表了在复杂生物流体中实时监测低分子量蛋白质领域的重大进展。
