Dorus Steve, Busby Scott A, Gerike Ursula, Shabanowitz Jeffrey, Hunt Donald F, Karr Timothy L
Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
Nat Genet. 2006 Dec;38(12):1440-5. doi: 10.1038/ng1915. Epub 2006 Nov 12.
In addition to delivering a haploid genome to the egg, sperm have additional critical functions, including egg activation, origination of the zygote centrosome and delivery of paternal factors. Despite this, existing knowledge of the molecular basis of sperm form and function is limited. We used whole-sperm mass spectrometry to identify 381 proteins of the Drosophila melanogaster sperm proteome (DmSP). This approach identified mitochondrial, metabolic and cytoskeletal proteins, in addition to several new functional categories. We also observed nonrandom genomic clustering of sperm genes and underrepresentation on the X chromosome. Identification of widespread functional constraint on the proteome indicates that sexual selection has had a limited role in the overall evolution of D. melanogaster sperm. The relevance of the DmSP to the study of mammalian sperm function and fertilization mechanisms is demonstrated by the identification of substantial homology between the DmSP and proteins of the mouse axoneme accessory structure.
除了将单倍体基因组传递给卵子外，精子还具有其他关键功能，包括卵子激活、受精卵中心体的形成以及父本因子的传递。尽管如此，目前关于精子形态和功能分子基础的知识仍然有限。我们使用全精子质谱法鉴定了黑腹果蝇精子蛋白质组（DmSP）中的381种蛋白质。这种方法除了鉴定出几个新的功能类别外，还鉴定出线粒体、代谢和细胞骨架蛋白。我们还观察到精子基因的非随机基因组聚类以及在X染色体上的低表达。对蛋白质组广泛功能限制的鉴定表明，性选择在黑腹果蝇精子的整体进化中作用有限。通过鉴定DmSP与小鼠轴丝附属结构蛋白之间的大量同源性，证明了DmSP与哺乳动物精子功能和受精机制研究的相关性。
