Halbhuber K J, Oehring H, Gliesing M, Stibenz D
Department of Histochemistry, Friedrich Schiller University, Jena, GDR.
Cell Mol Biol. 1990;36(6):643-57.
The value of membrane anisotropy after fixation and topo-optical analysis of erythrocytes stained with toluidine blue is a measure for the degree of spatial order of the dyestuff-binding acidic residues of the glycocalyx and thus a parameter for the characterization of the glycocalyx structure. Lowering the pH value and/or the ionic strength of the staining medium results in a decrease of membrane anisotropy indicating a lower order of the anionic residues. In agreement with the findings of other authors this phenomenon seems to be connected with an expansion of the glycocalyx. Diamide-induced oxidative crosslinking of spectrin before fixation and staining with toluidine blue at physiological pH and ionic strength also results in a decreased anisotropy. This indirect influence on the glycocalyx structure may be caused by an increase of the charge density within the glycocalyx due to a diamide-induced rearrangement of the membrane skeleton and of the transmembrane proteins bound to it.
用甲苯胺蓝染色的红细胞经固定和拓扑光学分析后，膜各向异性的值是糖萼中与染料结合的酸性残基空间有序程度的一种度量，因此是表征糖萼结构的一个参数。降低染色介质的pH值和/或离子强度会导致膜各向异性降低，表明阴离子残基的有序程度降低。与其他作者的研究结果一致，这种现象似乎与糖萼的扩张有关。在生理pH值和离子强度下，用甲苯胺蓝固定和染色前，二酰胺诱导的血影蛋白氧化交联也会导致各向异性降低。这种对糖萼结构的间接影响可能是由于二酰胺诱导的膜骨架和与其结合的跨膜蛋白重排，导致糖萼内电荷密度增加所致。
