Herrmann A, Lassmann G, Groth T, Donath E, Hillebrecht B
Biochim Biophys Acta. 1986 Sep 25;861(1):111-21. doi: 10.1016/0005-2736(86)90377-9.
The structure of the glycocalyx of the membrane of human erythrocytes and spectrin-depleted vesicles was studied under various conditions by two spin-labelling approaches: covalently labelling sialic acid residues of the glycocalyx and incorporation of a charged hydrophobic spin probe, CAT 16, being sensitive to alterations on the membrane surface into the lipid phase. Although cell electrophoretic measurements which were performed, additionally, indicated an erection of the glycocalyx upon decreasing the ionic strength of the suspension medium a more restricted mobility of spin-labelled sialic acid residues was found, in this case probably due to electrostatic interactions. The enhanced mobility of the spin probe CAT 16 at low ionic strength as well as in the case of neuraminidase-treated cells could be caused by reduced steric and electrostatic interaction with glycoproteins and glycolipids. La3+ adsorption and virus attachment on the human erythrocyte membrane were accompanied with a reduced mobility of sugar headgroups of the surface coat. No indication of cluster formation or lateral segregation of glycophorin molecules was found upon virus binding. After denaturation of the spectrin cytoskeleton of intact erythrocytes, increased mobility of spin-labelled sialic acid residues was observed.
采用两种自旋标记方法，在不同条件下研究了人红细胞膜和血影蛋白缺失囊泡糖萼的结构：共价标记糖萼的唾液酸残基，以及将对膜表面变化敏感的带电荷疏水自旋探针CAT 16掺入脂质相。此外，虽然进行的细胞电泳测量表明，降低悬浮介质的离子强度会使糖萼竖起，但在这种情况下，发现自旋标记的唾液酸残基迁移率更受限，这可能是由于静电相互作用。自旋探针CAT 16在低离子强度下以及在神经氨酸酶处理的细胞中迁移率增强，可能是由于与糖蛋白和糖脂的空间位阻和静电相互作用降低所致。La3 +在人红细胞膜上的吸附和病毒附着伴随着表面糖被糖头基团迁移率的降低。病毒结合后未发现糖蛋白分子形成簇或横向分离的迹象。完整红细胞的血影蛋白细胞骨架变性后，观察到自旋标记的唾液酸残基迁移率增加。
