Halbhuber K J, Stibenz D, Meyer H W, Zimmermann N, Linss W, Oehring H, Brox D
Biomed Biochim Acta. 1987;46(2-3):S88-92.
IgG receptor expression after selective cross-linking of spectrin by means of diamide was investigated. A diamide concentration dependent IgG loading of erythrocytes was observed. Furthermore, diamide causes disturbance of lipid asymmetry, decrease of the anisotropy after topooptical staining of the glycocalyx, increase of the phagocytosis index and aggregation of the IMP's. Our findings support the hypothesis that the arrangement of the membrane skeleton at the inner aspect of the membrane is decisive not only for the lipid asymmetry but also for the spatial structure of the glycocalyx at the outer aspect of the membrane and thus for the degree of exposure and arrangement of IgG-receptors, which are thought to be localized at an extracellular portion of band 3 protein.
研究了通过二酰胺选择性交联血影蛋白后IgG受体的表达。观察到二酰胺浓度依赖性的红细胞IgG负载。此外，二酰胺会导致脂质不对称性紊乱、糖萼拓扑光学染色后各向异性降低、吞噬指数增加以及整合膜蛋白聚集。我们的研究结果支持这样的假设，即膜骨架在膜内侧的排列不仅对脂质不对称性起决定性作用，而且对膜外侧糖萼的空间结构起决定性作用，进而对IgG受体的暴露程度和排列起决定性作用，据认为IgG受体定位于带3蛋白的细胞外部分。
