Pharmacognosy Department, Faculty of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Nat Prod Res. 2010 Oct;24(16):1537-45. doi: 10.1080/14786419.2010.489049.
Many species belonging to Cucurbitaceae family have long been regarded as food, medicinal plants, or both. Preliminary phytochemical screening of Citrullus colocynthis L., Cucumis sativus L. and Momordica charantia L. herbs showed the presence of phenolic compounds. Quantitative analysis of total phenolic compounds using Folin-Ciocalteu reagent revealed the presence of 50.87 mg GAE g(-1), 56.58 mg GAE g(-1) and 42.36 mg GAE g(-1) in C. colocynthis L., C. sativus L. and M. charantia L. herbs, respectively. HPLC analysis of phenolic content showed the presence of chlorogenic acid (16.3 mg per 100g dry sample and 27.7 mg per 100 g(-1) dry samples in C. colocynthis L. and C. sativus L., respectively) and gallic acid (26.7 mg per 100 g dry sample) as a major phenolic acids in M. charantia L. herb. The antioxidant activity of the herb of plants under investigation was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and total antioxidant capacity was determined in terms of GAE. This study showed that C. sativus L. is the most active antioxidant, followed by C. colocynthis L., while M. charantia L. has the least antioxidant activity.
许多属于葫芦科的物种长期以来一直被视为食物、药用植物，或兼具两者。对苦瓜、黄瓜和苦瓜的初步植物化学筛选显示存在酚类化合物。使用 Folin-Ciocalteu 试剂对总酚类化合物进行定量分析，结果表明苦瓜、黄瓜和苦瓜中的总酚类化合物含量分别为 50.87、56.58 和 42.36 mg GAE g(-1)。HPLC 分析酚类含量显示，苦瓜和黄瓜中分别存在绿原酸（16.3 mg/100g 干样品和 27.7 mg/100 g(-1)干样品）和没食子酸（26.7 mg/100 g 干样品）作为主要的酚酸。用 2,2-二苯基-1-苦基肼（DPPH）法评价了所研究植物的草药抗氧化活性，并以 GAE 表示总抗氧化能力。本研究表明，黄瓜是最活跃的抗氧化剂，其次是苦瓜，而苦瓜的抗氧化活性最低。
