Institute of Biological Chemistry and Nutrition, University of Hohenheim, Stuttgart, Germany.
Free Radic Res. 2010 Oct;44(10):1203-15. doi: 10.3109/10715762.2010.499907.
Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F(2)-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.
脂质过氧化产物，如丙二醛、4-羟壬烯醛和 F(2)-异前列腺素，被广泛用作体外和体内氧化应激的标志物。本研究报告了 COST 行动 B35 进行的多实验室验证研究的结果，以评估脂质过氧化测量中的实验室间和实验室内变异。将人血浆样品暴露于不同剂量（0、15 J、20 J）的 UVA 辐射下，进行编码并运送到 15 个实验室，在这些实验室中进行丙二醛、4-羟壬烯醛和异前列腺素的分析。结果表明，日内变化较小，在两天不同时间观察到的结果相关性良好。然而，实验室之间的变异系数很高。在 UVA 处理后，通过 HPLC 测定的丙二醛被发现是血浆中最敏感和可重复的脂质过氧化产物。因此，尽管承认这可能不适用于生物学有效性，但 HPLC 测定丙二醛用于人类 EDTA 血浆样品中脂质过氧化的实验室间研究具有良好的分析有效性。
