Moore K, Roberts L J
Department of Medicine, Royal Free Hospital School of Medicine, London, UK.
Free Radic Res. 1998 Jun;28(6):659-71. doi: 10.3109/10715769809065821.
Lipid peroxidation results in the formation of conjugated dienes, lipid hydroperoxides and degradation products such as alkanes, aldehydes and isoprostanes. The approach to the quantitative assessment of lipid peroxidation depends on whether the samples involve complex biological material obtained in vivo, or whether the samples involve relatively simple mixtures obtained in vitro. Samples obtained in vivo contain a large number of products which themselves may undergo metabolism. The measurement of conjugated diene formation is generally applied as a dynamic quantitation e.g. during the oxidation of LDL, and is not generally applied to samples obtained in vivo. Lipid hydroperoxides readily decompose, but can be measured directly and indirectly by a variety of techniques. The measurement of MDA by the TBAR assay is non-specific, and is generally poor when applied to biological samples. More recent assays based on the measurement of MDA or HNE-lysine adducts are likely to be more applicable to biological samples, since adducts of these reactive aldehydes are relatively stable. The discovery of the isoprostanes as lipid peroxidation products which can be measured by gas chromatography mass spectrometry or immunoassay has opened a new avenue by which to quantify lipid peroxidation in vivo, and will be discussed in detail.
脂质过氧化作用会导致共轭二烯、氢过氧化物以及降解产物（如烷烃、醛类和异前列腺素）的形成。脂质过氧化作用的定量评估方法取决于样品是来自体内获取的复杂生物材料，还是来自体外获取的相对简单的混合物。体内获取的样品包含大量自身可能会发生代谢的产物。共轭二烯形成的测量通常用作动态定量，例如在低密度脂蛋白氧化过程中，一般不适用于体内获取的样品。氢过氧化物容易分解，但可以通过多种技术直接或间接测量。通过硫代巴比妥酸反应物（TBAR）法测量丙二醛不具有特异性，应用于生物样品时通常效果不佳。基于丙二醛或HNE-赖氨酸加合物测量的最新检测方法可能更适用于生物样品，因为这些反应性醛类的加合物相对稳定。异前列腺素作为脂质过氧化产物被发现，可通过气相色谱-质谱联用或免疫测定法进行测量，这为体内脂质过氧化作用的定量开辟了一条新途径，将进行详细讨论。
