Programa de Pós-Graduação em Ciências Farmacêuticas, Porto Alegre, RS, Brazil.
Metab Brain Dis. 2010 Sep;25(3):297-304. doi: 10.1007/s11011-010-9211-0. Epub 2010 Sep 14.
Diabetes may modify central nervous system functions and is associated with moderate cognitive deficits and changes in the brain, a condition that may be referred to as diabetic encephalopathy. The prevalence of depression in diabetic patients is higher than in the general population, and clonazepam is being used to treat this complication. Oxidative stress may play a role in the development of diabetes complications. We investigated oxidative stress parameters in streptozotocin-induced diabetic rats submitted to forced swimming test (STZ) and evaluated the effect of insulin (STZ-INS) and/or clonazepam (STZ-CNZ and STZ-INS-CNZ) acute treatment on these animal model. Oxidative damage to proteins measured as carbonyl content in plasma was significantly increased in STZ group compared to STZ treated groups. Malondialdehyde plasma levels were significantly reduced in STZ-INS and STZ-INS-CNZ groups when compared to STZ rats, being significantly reduced in STZ-INS-CNZ than STZ-INS rats. The activities of the antioxidant enzymes catalase, superoxide dismutase and glutathione peroxidase showed no significant differences among all groups of animals. These findings showed that protein and lipid damage occurs in this diabetes/depression animal model and that the associated treatment of insulin and clonazepam is capable to protect against oxidative damage in this experimental model.
糖尿病可能会改变中枢神经系统的功能，与中度认知功能障碍和大脑变化有关，这种情况可能被称为糖尿病性脑病。糖尿病患者的抑郁患病率高于一般人群，且正在使用氯硝西泮来治疗这种并发症。氧化应激可能在糖尿病并发症的发展中起作用。我们研究了链脲佐菌素诱导的糖尿病大鼠在强迫游泳试验（STZ）中氧化应激参数，并评估了胰岛素（STZ-INS）和/或氯硝西泮（STZ-CNZ 和 STZ-INS-CNZ）急性治疗对这些动物模型的影响。与 STZ 治疗组相比，STZ 组大鼠血浆中的蛋白羰基含量明显增加，表明氧化损伤增加。与 STZ 大鼠相比，STZ-INS 和 STZ-INS-CNZ 组大鼠的血浆丙二醛水平明显降低，且 STZ-INS-CNZ 组比 STZ-INS 组降低更明显。抗氧化酶过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶的活性在所有动物组之间均无显著差异。这些发现表明，在这种糖尿病/抑郁动物模型中发生了蛋白和脂质损伤，而胰岛素和氯硝西泮的联合治疗能够在该实验模型中预防氧化损伤。
