Programa de Pós-Graduação em Ciências Farmacêuticas , Porto Alegre, RS , Brasil.
Pharm Biol. 2013 May;51(5):533-8. doi: 10.3109/13880209.2012.747544. Epub 2013 Feb 1.
It is known that oxidative stress occurs in peripheral blood in an experimental animal model of diabetes and depression, and acute treatment with insulin and clonazepam (CNZ) has a protective effect on oxidative stress in this model.
This study evaluated the effect of insulin plus CNZ on oxidative stress parameters in the liver of diabetic male rats induced with streptozotocin (STZ) and subjected to forced swimming test (FST).
Diabetes was induced by a single intraperitoneal (i.p.) dose of STZ 60 mg/kg in male Wistar rats. Insulin (4 IU/kg) plus CNZ acute i.p. treatment (0.25 mg/kg) was administered 24, 5 and 1 h before the FST. Nondiabetic control rats received i.p. injections of saline (1 mL/kg). Protein oxidative damage was evaluated by carbonyl formation and the antioxidant redox parameters were analyzed by the measurements of enzymatic activities of the superoxide dismutase (SOD), catalase and glyoxalase I (GLO). Glycemia levels also were determined.
Our present study has shown an increase in carbonyl content from diabetic rats subjected to FST (2.04 ± 0.55), while the activity of catalase (51.83 ± 19.02) and SOD (2.30 ± 1.23) were significantly decreased in liver from these animals, which were reverted by the treatment. Also, the activity of GLO (0.15 ± 0.02) in the liver of the animals was decreased.
Our findings showed that insulin plus CNZ acute treatment ameliorate the antioxidant redox parameters and protect against protein oxidative damage in the liver of diabetic rats subjected to FST.
已知糖尿病和抑郁症实验动物模型的外周血中存在氧化应激，胰岛素和氯硝西泮（CNZ）的急性治疗对该模型的氧化应激具有保护作用。
本研究评估了胰岛素加 CNZ 对链脲佐菌素（STZ）诱导的糖尿病雄性大鼠肝脏氧化应激参数的影响，这些大鼠还进行了强迫游泳试验（FST）。
雄性 Wistar 大鼠一次性腹腔（i.p.）注射 STZ60mg/kg 诱导糖尿病。FST 前 24、5 和 1 小时，给予胰岛素（4IU/kg）加 CNZ 急性 i.p.治疗（0.25mg/kg）。非糖尿病对照大鼠接受 i.p.注射生理盐水（1mL/kg）。通过羰基形成评估蛋白质氧化损伤，通过超氧化物歧化酶（SOD）、过氧化氢酶和乙二醛酶 I（GLO）的酶活性分析来分析抗氧化剂氧化还原参数。还测定了血糖水平。
本研究表明，进行 FST 的糖尿病大鼠的羰基含量增加（2.04±0.55），而这些动物肝脏中的过氧化氢酶（51.83±19.02）和 SOD（2.30±1.23）活性显著降低，这种情况通过治疗得到了逆转。此外，动物肝脏中的 GLO 活性（0.15±0.02）降低。
我们的研究结果表明，胰岛素加 CNZ 急性治疗可改善 FST 后糖尿病大鼠的抗氧化剂氧化还原参数，并可防止其肝脏的蛋白质氧化损伤。
