Assreuy Ana Maria S, Pontes Grazielle C, Rodrigues Natália V F C, Gomes Daniel M, Xavier Paulo A, Araujo Glacio S, Sampaio Alexandre H, Cavada Benildo S, Pereira Maria G, Farias Wladimir R L
Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana 1700, 60.740-000, Fortaleza-CE-Brazil.
Nat Prod Commun. 2010 Aug;5(8):1267-72.
Anticoagulant and antithrombotic properties of sulfated-polysaccharides (SP) from marine algae are extensively exploited. However, reports on the vascular effects of SP from red algae are rare in the literature. The polysaccharide from Solieria filiformis (Sf-SP) was isolated by ion exchange chromatography, analyzed by agarose gel electrophoresis and tested in male Wistar rats. The inflammation studies were performed using the paw-edema model and the relaxant activity in isolated aorta pre-contracted with phenylephrine. The anticoagulant effect was evaluated by the test of partial thromboplastin activation time. The SP (1 mg/kg) was not anti-inflammatory, but induced acute edema with maximal activity at 30 min (0.35 +/- 0.04 mL) compared to controls (0.05 +/- 0.03 mL). Cumulative addition of Sf-SP in phenylephrine-contracted tissues produced relaxation with maximal inhibition of 69% (IC50 29.3 +/- 9.0 microg/mL) at 300 microg/mL in comparison to controls (0.51 +/- 0.09 g). Sf-SP also extended human plasma coagulation time by 2.1 times. These substances could be used as important tools for the study of vascular alterations.
海藻硫酸化多糖（SP）的抗凝血和抗血栓特性得到了广泛应用。然而，文献中关于红藻来源的SP对血管影响的报道却很少。丝状索藻多糖（Sf-SP）通过离子交换色谱法分离，经琼脂糖凝胶电泳分析，并在雄性Wistar大鼠身上进行测试。采用爪肿胀模型进行炎症研究，并检测其对苯肾上腺素预收缩的离体主动脉的舒张活性。通过部分凝血活酶激活时间试验评估抗凝作用。SP（1毫克/千克）没有抗炎作用，但与对照组（0.05±0.03毫升）相比，在30分钟时诱导急性水肿，最大活性为0.35±0.04毫升。在苯肾上腺素收缩的组织中累积添加Sf-SP可产生舒张作用，在300微克/毫升时最大抑制率为69%（IC50为29.3±9.0微克/毫升），而对照组为0.51±0.09克。Sf-SP还可使人体血浆凝血时间延长2.1倍。这些物质可作为研究血管改变的重要工具。
