Department of Physiology, College of Oriental Medicine Department of Oriental Pathology, College of Oriental Medicine, KyungHee University, Seoul 130-701, Republic of Korea.
Br J Clin Pharmacol. 2010 Oct;70(4):547-56. doi: 10.1111/j.1365-2125.2010.03724.x.
The rhizome of turmeric, Curcuma longa (CL), is a herbal medicine used in many traditional prescriptions. It has previously been shown that CL treatment showed greater than 47% recovery from cisplatin-induced cell damage in human kidney HEK 293 cells. This study was conducted to evaluate the recovery mechanisms of CL that occur during cisplatin induced nephrotoxicity by examining the genome wide mRNA expression profiles of HEK 293 -cells.
Recovery mechanisms of CL that occur during cisplatin-induced nephrotoxicity were determined by microarray, real-time PCR, immunofluorescent confocal microscopy and Western blot analysis.
The results of microarray analysis and real-time PCR revealed that NFκB pathway-related genes and apoptosis-related genes were down-regulated in CL-treated HEK 293 cells. In addition, immunofluorescent confocal microscopy and Western blot analysis revealed that NFκB p65 nuclear translocation was inhibited in CL-treated HEK 293 cells. Therefore, the mechanism responsible for the effects of CL on HEK 293 cells is closely associated with regulation of the NFκB pathway.
CL possesses novel therapeutic agents that can be used for the prevention or treatment of cisplatin-induced renal disorders.
姜黄的根茎，姜黄（CL），是一种草药，用于许多传统处方。先前已经表明，CL 治疗在人肾 HEK 293 细胞中显示出超过 47%的顺铂诱导的细胞损伤的恢复。本研究通过检查 HEK 293-细胞中顺铂诱导的肾毒性过程中的全基因组 mRNA 表达谱，评估 CL 在顺铂诱导的肾毒性过程中发生的恢复机制。
通过微阵列、实时 PCR、免疫荧光共聚焦显微镜和 Western blot 分析确定 CL 在顺铂诱导的肾毒性过程中的恢复机制。
微阵列分析和实时 PCR 的结果表明，CL 处理的 HEK 293 细胞中 NFκB 通路相关基因和凋亡相关基因下调。此外，免疫荧光共聚焦显微镜和 Western blot 分析表明，CL 处理的 HEK 293 细胞中 NFκB p65 核易位受到抑制。因此，CL 对 HEK 293 细胞的作用机制与 NFκB 通路的调节密切相关。
CL 具有新的治疗剂，可用于预防或治疗顺铂诱导的肾脏疾病。
