BK21 Oriental Medical Science Center, KyungHee University, Seoul 130-701, Republic of Korea.
Environ Toxicol Pharmacol. 2009 Sep;28(2):206-12. doi: 10.1016/j.etap.2009.04.005. Epub 2009 Apr 15.
The goal of this study was to quantitatively determine the recovery effects of herbal medicines (HM) on the cisplatin-induced nephrotoxicity. In the present study, the recovery effects of 239 HM on HEK 293 cells that had been damaged by cisplatin were evaluated by a mitochondrial activity MTS assay. After the first round of screening, candidate HM were selected based on a recovery rate of greater than 20%. The efficacy of the selected herbs was then determined by dose response kinetic analysis. Of the extracts evaluated, 7 HM (Paeonia suffruticosa (PS), Curcuma longa (CL), Centipeda minima (CM), Loranthus parasiticus (LP), Pulsatilla dahurica (PD), Sinapis alba (SA), and Scutellaria barbata (SB)) had a strong recovery effect on cisplatin-induced damage in HEK 293 cells. An LDH assay showed that LP, CM, SB, CL, SA, and PS had the best recovery effect, whereas a comet assay indicated that PS, SB, SA, PD, and CL had the best recovery effect. Taken together, these results suggest that SB, CL, PS, and SA are the best candidate HM for the recovery of cisplatin-induced nephrotoxicity. Therefore, additional studies should be conducted to determine if these HM possess novel therapeutic agents that can be used for the prevention or treatment of renal disorders.
本研究旨在定量评估草药（HM）对顺铂诱导的肾毒性的恢复作用。在本研究中，通过线粒体活性 MTS 测定法评估了 239 种 HM 对顺铂损伤的 HEK 293 细胞的恢复作用。经过第一轮筛选，根据恢复率大于 20%选择候选 HM。然后通过剂量反应动力学分析确定所选草药的疗效。在所评估的提取物中，7 种 HM（牡丹皮（PS）、姜黄（CL）、小飞扬草（CM）、桑寄生（LP）、白头翁（PD）、白芥子（SA）和白花蛇舌草（SB））对 HEK 293 细胞的顺铂诱导损伤具有很强的恢复作用。LDH 测定表明 LP、CM、SB、CL、SA 和 PS 具有最佳的恢复效果，而彗星试验表明 PS、SB、SA、PD 和 CL 具有最佳的恢复效果。综上所述，这些结果表明 SB、CL、PS 和 SA 是恢复顺铂诱导肾毒性的最佳候选 HM。因此，应进行进一步的研究以确定这些 HM 是否具有可用于预防或治疗肾脏疾病的新型治疗剂。
