Department of Physiology, College of Oriental Medicine, Kyung Hee University, 1, Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Environ Toxicol Pharmacol. 2009 Nov;28(3):453-8. doi: 10.1016/j.etap.2009.08.001. Epub 2009 Aug 11.
The root cortex of Paeonia suffruticosa Andrews (PS) is a crude Chinese drug used in many traditional prescriptions. This study was conducted to evaluate the recovery mechanisms induced by PS in HEK 293 cells treated with cisplatin. Previous studies revealed that 1μg/ml PS treatment showed greater than 20% recovery of 14μM cisplatin-induced 50% loss of cell proliferation (IC(50)) determined by mitochondrial activity MTS assay in HEK 293 cells (Sohn et al., 2009). In the present study, the effects of PS on gene expression profiles in stimulated HEK 293 cells were evaluated by oligonucleotide microarray and real-time RT-PCR. The results of the microarray analysis and real-time RT-PCR revealed that DNA repair- and cell proliferation-related genes were up-regulated in PS-treated HEK 293 cells. The mechanism responsible for the effects of PS on HEK 293 cells is closely associated with the regulation of DNA repair and cell proliferation. Thus, PS possesses novel therapeutic potential that could be used for the prevention or treatment of cisplatin-induced nephrotoxicity.
牡丹皮的根皮层是一种在许多传统方剂中使用的粗制中药。本研究旨在评估牡丹皮在顺铂处理的 HEK 293 细胞中诱导的恢复机制。先前的研究表明，1μg/ml 的牡丹皮处理显示出大于 20%的恢复，由线粒体活性 MTS 测定确定的 14μM 顺铂诱导的 50%细胞增殖丧失（IC50）（Sohn 等人，2009）。在本研究中，通过寡核苷酸微阵列和实时 RT-PCR 评估了牡丹皮对刺激的 HEK 293 细胞中基因表达谱的影响。微阵列分析和实时 RT-PCR 的结果表明，牡丹皮处理的 HEK 293 细胞中与 DNA 修复和细胞增殖相关的基因上调。牡丹皮对 HEK 293 细胞的作用机制与 DNA 修复和细胞增殖的调节密切相关。因此，牡丹皮具有新的治疗潜力，可用于预防或治疗顺铂引起的肾毒性。
