Section of Cartilage Regeneration, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
BMC Musculoskelet Disord. 2010 Sep 14;11:208. doi: 10.1186/1471-2474-11-208.
Though sera are essential for Mesenchymal stem cells (MSCs), the effect of heat-inactivation remains unknown. Autologous human serum is recommended for clinical use; however, it is unclear whether differentiation potentials are maintained. To examine whether heat-inactivation of serum affected the proliferation and whether autologous human serum influenced their multipotentiality.
After whole blood collection, human synovium and bone marrow were harvested. Nucleated cells were expanded with autologous human serum and FBS.
Heat-inactivation of autologous human serum enhanced proliferation of synovial MSCs. Heat-inactivation of each types of serum didn't affect calcification of synovial MSCs. The induction of calcification increased ALP activity, with the exception of bone marrow MSCs with autologous human serum. For adipogenesis of synovial MSCs, the Oil Red-O positive colony forming efficiency with autologous human serum was similar to or less than that with FBS.
These clarified the processing of human autologous serum and the influence of different sera for differentiation of synovial and bone marrow MSCs.
间充质干细胞（MSCs）的培养需要用到血清，但是热灭活处理对其影响目前尚不清楚。临床上推荐使用自体人血清，但是否能维持其多能性还不清楚。本研究旨在观察血清的热灭活处理是否会影响细胞的增殖，以及自体人血清是否会影响其多能性。
采集全血后，分别获取滑膜和骨髓组织。利用自体人血清和胎牛血清（FBS）对核细胞进行扩增。
自体人血清的热灭活处理可增强滑膜间充质干细胞的增殖能力。热灭活处理对滑膜间充质干细胞的钙化无影响。除了骨髓间充质干细胞用自体人血清诱导钙化会增加碱性磷酸酶（ALP）活性外，其他类型的血清都没有影响。对于滑膜间充质干细胞的成脂分化，油红 O 阳性集落形成效率与 FBS 相比，自体人血清的效果相似或更差。
本研究阐明了人自体血清的处理方法，以及不同血清对滑膜和骨髓间充质干细胞分化的影响。
