Department of Chemistry Texas A&M University, College Station, TX 77843, USA.
Bioorg Med Chem Lett. 2010 Nov 1;20(21):6321-3. doi: 10.1016/j.bmcl.2010.07.046. Epub 2010 Jul 15.
A retro-inverso, TAT-like peptide wherein lysine residues are replaced with cysteine residues bearing a disulfide-linked cysteamine group is found to engage in thiol-disulfide exchange with cysteine. These peptides are transported into cells and localize to lysosomes. Cellular uptake is enhanced in peptides bearing two cysteamine groups over those with one or none, by factors of approximately 1.5 and 12, respectively.
一种具有反向重复序列和 TAT 样结构的肽，其中赖氨酸残基被带有二硫键连接的半胱胺基团的半胱氨酸残基取代，被发现与半胱氨酸进行巯基-二硫键交换。这些肽被转运到细胞内，并定位于溶酶体。带有两个半胱胺基团的肽比带有一个或没有半胱胺基团的肽的细胞摄取分别增强了约 1.5 倍和 12 倍。
