Zacharopoulou Antigone, Augustinos Antonios A, Sayed Waheed A A, Robinson Alan S, Franz Gerald
Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Agency's Laboratories, Seibersdorf, Austria.
Genetica. 2011 Jan;139(1):79-90. doi: 10.1007/s10709-010-9495-3. Epub 2010 Sep 16.
The Oriental fruit fly, Batrocera dorsalis s.s. (Hendel) is one of the most destructive agricultural pests, belonging to a large group of difficult to distinguish morphologically species, referred as the B. dorsalis complex. We report here a cytogenetic analysis of two laboratory strains of the species and provide a photographic polytene chromosome map from larval salivary glands. The mitotic complement consists of six chromosome pairs including a heteromorphic sex (XX/XY) chromosome pair. Analysis of the polytene complement has shown a total of five polytene chromosomes (10 polytene arms) that correspond to the five autosomes. The most important landmarks of each polytene chromosome and characteristic asynapsis at a specific chromosomal region are presented and discussed. Chromosomal homology between B. dorsalis and Ceratitis capitata has been determined by comparing chromosome banding patterns. The detection of chromosome inversions in both B. dorsalis strains is shown and discussed. Our results show that the polytene maps presented here are suitable for cytogenetic analysis of this species and can be used for comparative studies among species of the Tephritidae family. They also provide a diagnostic tool that could accelerate species identification within the B. dorsalis complex and could shed light on the ongoing speciation in this complex. Polytene chromosome maps can facilitate the development of biological control methods and support the genome mapping project of the species that is currently in progress.
东方果实蝇，即南亚果实蝇（Hendel），是最具破坏力的农业害虫之一，属于一大类形态上难以区分的物种，被称为南亚果实蝇复合种。我们在此报告对该物种两个实验室品系的细胞遗传学分析，并提供幼虫唾液腺的多线染色体图谱照片。有丝分裂染色体组由六对染色体组成，包括一对异形性染色体（XX/XY）。对多线染色体组的分析显示共有五条多线染色体（10条多线臂），对应于五条常染色体。本文展示并讨论了每条多线染色体的最重要特征以及特定染色体区域的特征性联会紊乱。通过比较染色体带型确定了南亚果实蝇和地中海实蝇之间的染色体同源性。展示并讨论了在两个南亚果实蝇品系中检测到的染色体倒位。我们的结果表明，本文呈现的多线图谱适用于该物种的细胞遗传学分析，可用于实蝇科物种间的比较研究。它们还提供了一种诊断工具，可加速南亚果实蝇复合种内的物种鉴定，并有助于揭示该复合种正在进行的物种形成过程。多线染色体图谱有助于生物防治方法的开发，并支持目前正在进行的该物种基因组图谱绘制项目。
