Shahjahan Reza M, Yesmin Farzana
Institute of Food and Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), G.P.O. Box 3787, Dhaka 1000, Bangladesh.
Genome. 2002 Dec;45(6):1167-74. doi: 10.1139/g02-081.
Standard photographic maps of the polytene chromosomes are presented for the melon fly Bactrocera cucurbitae, a serious pest of fleshy fruits and vegetables. Five larval salivary gland polytene chromosomes (10 polytene arms) were isolated, and their characteristic features and landmarks have been recognized. Banding patterns of each of the polytene arms are presented, where variation in band intensity and puffs appear to reflect fundamental differences in chromosomes. The whole polytene genome has been typically mapped by dividing it into 100 sections and the subsections were lettered. The mitotic chromosomes of larval brain ganglia are also examined, five pairs of autosomes and an XX/XY sex chromosome pair. In addition, a heterochromatic mass corresponding to the sex chromosomes are observed in the polytene nuclei of salivary gland tissue. This investigation showed that B. cucurbitae has excellent cytological material for polytene chromosome analysis and proved to be very useful for obtaining more detailed genetic information on the pest's natural populations.
本文展示了瓜实蝇（Bactrocera cucurbitae）的多线染色体标准照片图谱，瓜实蝇是一种严重危害肉质水果和蔬菜的害虫。分离出了五条幼虫唾液腺多线染色体（10条多线臂），并识别出了它们的特征和标记。展示了每条多线臂的带型，其中带强度和胀泡的变化似乎反映了染色体的基本差异。整个多线基因组通常被划分为100个区段，并对这些子区段进行了字母标注。还检查了幼虫脑神经节的有丝分裂染色体，包括五对常染色体和一对XX/XY性染色体。此外，在唾液腺组织的多线核中观察到了与性染色体相对应的异染色质团。这项研究表明，瓜实蝇具有用于多线染色体分析的优良细胞学材料，并且被证明对于获取该害虫自然种群更详细的遗传信息非常有用。
