Molina W F, Alves D E O, Araújo W C, Martinez P A, Silva M F M, Costa G W W F
Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
Genet Mol Res. 2010 Sep 14;9(3):1807-14. doi: 10.4238/vol9-3gmr840.
Inoculation with bacterial or fungal antigens that stimulate cell proliferation has been widely used to obtain metaphases for cytogenetic studies of fish. We evaluated the potential of new pharmaceutical compounds as mitogenic agents in fish, testing the efficacy of Aminovac (mixed antigens and epsilon-acetamidocaproic acid), Broncho-Vaxom (bacterial lysate) and Estimoral (bacterial lysate) to increase the mitotic index in fingerlings of the Neotropical fish Prochilodus brevis (Prochilodontidae) and Hoplias malabaricus (Erythrinidae), which were obtained from an aquaculture facility. The animals were treated with intramuscular or intraperitoneal injections of 1 mL/50 g body weight of each compound. After 24 h, cytogenetic analyses were performed. All immunostimulants tested significantly stimulated cell division, although Aminovac proved to be the most efficient, leading to a 5-fold increase in the number of metaphase cells compared to the control group and to a 2-fold increase compared to conventional yeast inoculation. This compound facilitates fish cytogenetics analyses as it stimulates the proliferation of defense cells and reduces loss of samples. It will be especially useful for the study of specimens that either have a high commercial value or are fragile, small and/or rare.
接种能刺激细胞增殖的细菌或真菌抗原来获取中期分裂相，已被广泛用于鱼类的细胞遗传学研究。我们评估了新型药物化合物作为鱼类促有丝分裂剂的潜力，测试了氨基疫苗（混合抗原和ε-乙酰氨基己酸）、支气管疫苗（细菌裂解物）和免疫刺激素（细菌裂解物）在新热带鱼短盖巨脂鲤（脂鲤科）和马氏狗脂鲤（狗脂鲤科）幼鱼中提高有丝分裂指数的效果，这些幼鱼来自一家水产养殖设施。给动物肌肉注射或腹腔注射每一种化合物，剂量为1 mL/50 g体重。24小时后，进行细胞遗传学分析。所有测试的免疫刺激剂均显著刺激了细胞分裂，不过氨基疫苗被证明是最有效的，与对照组相比，中期细胞数量增加了5倍，与传统酵母接种相比增加了2倍。这种化合物促进了鱼类细胞遗传学分析，因为它刺激防御细胞增殖并减少样本损失。它对于研究具有高商业价值或脆弱、小型和/或稀有的标本将特别有用。
