Walter Schottky Institut, Technische Universität München, 85747 Garching, Germany.
Langmuir. 2010 Oct 19;26(20):15895-900. doi: 10.1021/la102225r.
A lithographic method to generate reactive thiol groups on functionalized synthetic diamond for biosensor and molecular electronic applications is developed. We demonstrate that ultrananocrystalline diamond (UNCD) thin films covalently functionalized with surface-generated thiol groups allow controlled thiol-disulfide exchange surface hybridization processes. The generation of the thiol functional head groups was obtained by irradiating phenylsulfonic acid (PSA) monolayers on UNCD surfaces. The conversion of the functional headgroup of the self-assembled monolayer was verified by using X-ray photoelectron spectroscopy (XPS), near-edge X-ray absorption fine structure (NEXAFS), and fluorescence microscopy. Our findings indicate the selective generation of reactive thiol surface groups. Furthermore, we demonstrate the grafting of yeast cytochrome c to the thiol-modified diamond surface and the electron transfer between protein and electrode.
我们开发了一种在功能化合成金刚石上生成反应性巯基的光刻方法，用于生物传感器和分子电子应用。我们证明，通过表面生成的巯基官能团共价功能化的纳米晶金刚石（UNCD）薄膜允许控制的硫醇-二硫化物交换表面杂交过程。通过在 UNCD 表面上辐照苯磺酸（PSA）单层，得到了巯基官能团的生成。通过 X 射线光电子能谱（XPS）、近边 X 射线吸收精细结构（NEXAFS）和荧光显微镜验证了自组装单层的官能团转化。我们的研究结果表明，选择性地生成了反应性巯基表面基团。此外，我们还证明了酵母细胞色素 c 接枝到巯基修饰的金刚石表面以及蛋白质和电极之间的电子转移。
