Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu, PR China .
DNA Cell Biol. 2011 Jan;30(1):3-8. doi: 10.1089/dna.2010.1058. Epub 2010 Sep 17.
The sugarcane borer, Diatraea saccharalis (F.), is an economically important pest of several major crops in North and South America. There is great concern over potential resistance development to the valuable transgenic Bacillus thuringiensis corn. Cost-effective monitoring methods, especially regarding molecular markers that could detect early changes in resistance allele frequency in field populations of D. saccharalis, are needed. This article reports the complete mitochondrial genome sequence of D. saccharalis (GenBank: FJ240227). The mitochondrial genome sequence of D. saccharalis has the typical metazoan mitogenome structure including 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes, with a total size of 15,490 bp. The A+T content of the full mitogenome is 80.1% and the significant A+T bias is at the control region (94.9%), as compared to the extremely low G content (0.6%). In all 13 protein coding genes, 9 start with the common Met initiator codon (ATA or ATG) and 3 use ATT (Ile), whereas CGA (Arg) is used as a start codon in COI. Eleven of the 13 protein coding genes use complete termination codon (TAA), whereas COI and COII use incomplete ones, which terminated with a single T-nucleotide abutting on tRNA. There are seven major noncoding spacers (628 bp), including six intergenic spacers and an A+T-rich region, which are scattered in the mitogenome. Several microsatellite-like elements were observed in these noncoding regions. The complete mitochondrial sequences of D. saccharalis reported in this study can provide useful data in analyzing divergence of lepidopteran insects and in developing DNA-based diagnoses and genetic makers.
甘蔗钻心虫，Diatraea saccharalis（F.），是北美洲和南美洲几种主要作物的重要经济害虫。人们非常担心宝贵的转基因苏云金芽孢杆菌玉米可能会产生抗药性。需要经济有效的监测方法，特别是关于分子标记的方法，这些方法可以检测到田间种群中 D. saccharalis 抗性等位基因频率的早期变化。本文报道了 D. saccharalis（GenBank：FJ240227）的完整线粒体基因组序列。D. saccharalis 的线粒体基因组序列具有典型的后生动物线粒体基因组结构，包括 13 个蛋白质编码基因、2 个 rRNA 基因和 22 个 tRNA 基因，总大小为 15490bp。整个线粒体基因组的 A+T 含量为 80.1%，而控制区的 A+T 偏倚非常明显（94.9%），与极低的 G 含量（0.6%）形成鲜明对比。在所有 13 个蛋白质编码基因中，9 个以常见的 Met 起始密码子（ATA 或 ATG）开始，3 个以 ATT（Ile）开始，而 COI 以 CGA（Arg）开始。13 个蛋白质编码基因中的 11 个使用完整的终止密码子（TAA），而 COI 和 COII 使用不完整的终止密码子，即在 tRNA 上以单个 T 核苷酸终止。有七个主要的非编码间隔区（628bp），包括六个基因间间隔区和一个富含 A+T 的区域，散布在整个线粒体基因组中。在这些非编码区域中观察到了几个微卫星样元件。本研究报道的 D. saccharalis 完整线粒体序列可为分析鳞翅目昆虫的分化以及开发 DNA 诊断和遗传标记提供有用数据。
