Sun Yu, Chen Chen, Gao Jin, Abbas Muhammad Nadeem, Kausar Saima, Qian Cen, Wang Lei, Wei Guoqing, Zhu Bao-Jian, Liu Chao-Liang
College of Life Sciences, Anhui Agricultural University, Hefei, China.
PLoS One. 2017 Jun 9;12(6):e0178773. doi: 10.1371/journal.pone.0178773. eCollection 2017.
In the present study, the complete sequence of the mitochondrial genome (mitogenome) of Daphnis nerii (Lepidoptera: Sphingidae) is described. The mitogenome (15,247 bp) of D.nerii encodes13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and an adenine (A) + thymine (T)-rich region. Its gene complement and order is similar to that of other sequenced lepidopterans. The 12 PCGs initiated by ATN codons except for cytochrome c oxidase subunit 1 (cox1) gene that is seemingly initiated by the CGA codon as documented in other insect mitogenomes. Four of the 13 PCGs have the incomplete termination codon T, while the remainder terminated with the canonical stop codon. This mitogenome has six major intergenic spacers, with the exception of A+T-rich region, spanning at least 10 bp. The A+T-rich region is 351 bp long, and contains some conserved regions, including 'ATAGA' motif followed by a 17 bp poly-T stretch, a microsatellite-like element (AT)9 and also a poly-A element. Phylogenetic analyses based on 13 PCGs using maximum likelihood (ML) and Bayesian inference (BI) revealed that D. nerii resides in the Sphingidae family.
在本研究中，描述了烟草天蛾（鳞翅目：天蛾科）线粒体基因组（mitogenome）的完整序列。烟草天蛾的线粒体基因组（15,247 bp）编码13个蛋白质编码基因（PCGs）、22个转运RNA基因（tRNAs）、两个核糖体RNA基因（rRNAs）和一个富含腺嘌呤（A）+胸腺嘧啶（T）的区域。其基因组成和顺序与其他已测序的鳞翅目昆虫相似。12个PCGs由ATN密码子起始，除了细胞色素c氧化酶亚基1（cox1）基因，该基因似乎如其他昆虫线粒体基因组中所记载的那样由CGA密码子起始。13个PCGs中有4个具有不完整的终止密码子T，其余的则以典型的终止密码子结束。该线粒体基因组有6个主要的基因间隔区，除了富含A+T的区域外，跨度至少为10 bp。富含A+T的区域长351 bp，包含一些保守区域，包括“ATAGA”基序，其后跟着一个17 bp的多聚T序列、一个微卫星样元件（AT）9以及一个多聚A元件。基于13个PCGs使用最大似然法（ML）和贝叶斯推断（BI）进行的系统发育分析表明，烟草天蛾属于天蛾科。
