All India Shri Shivaji Memorial Society's College of Pharmacy, Department of Pharmacology, Pune, Maharashtra, India.
Food Chem Toxicol. 2010 Dec;48(12):3412-7. doi: 10.1016/j.fct.2010.09.013. Epub 2010 Sep 16.
The hydroalcoholic extract of Areca catechu L. (ANE) nut was screened for its analgesic, anti-inflammatory and in vitro antioxidant potential. Three doses of ANE (250, 500 and 1000 mg/kg orally) were tested for analgesic and anti-inflammatory activities. Evaluation of analgesic activity of ANE was performed using hot plate and formalin test in mice. ANE showed maximum increase in hot plate reaction time (56.27%, p<0.01), while reduced the duration of licking/biting behaviors in first (39.45%, p<0.05) and second (92.71%, p<0.01) phases of the formalin test indicating significant analgesic activity. ANE reduced the paw edema considerably (86.79% inhibition after 24h, p<0.01) in dose-dependent manner compared to carrageenan-induced rat. In addition, in vitro antioxidant activity of ANE was investigated by total phenolic content (TPC) and hydrogen peroxide assay. The IC(50) observed in hydrogen peroxide assay was 83.14 μg/ml and TPC 120.56±21.09 mg QE/g. Altogether, these results suggest that the hydroalcoholic extract of Areca catechu could be considered as a potential analgesic, anti-inflammatory and antioxidant agent.
荖叶（Areca catechu L.）水醇提取物的镇痛、抗炎和体外抗氧化活性研究。荖叶水醇提取物（ANE）三个剂量（250、500 和 1000mg/kg 口服）用于镇痛和抗炎活性测试。荖叶的镇痛活性通过热板和福尔马林试验在小鼠中进行评估。ANE 最大程度地增加了热板反应时间（56.27%，p<0.01），同时减少了福尔马林试验中第一（39.45%，p<0.05）和第二（92.71%，p<0.01）阶段的舔/咬行为持续时间，表明具有显著的镇痛活性。与角叉菜胶诱导的大鼠相比，ANE 以剂量依赖性方式显著减少了足肿胀（24 小时后抑制率为 86.79%，p<0.01）。此外，还通过总酚含量（TPC）和过氧化氢测定法研究了 ANE 的体外抗氧化活性。过氧化氢试验中观察到的 IC50 为 83.14μg/ml，TPC 为 120.56±21.09mg QE/g。综上所述，这些结果表明荖叶水醇提取物可被视为一种有潜力的镇痛、抗炎和抗氧化剂。
