Sanpinit Sineenart, Wetchakul Palika, Chonsut Piriya, Prommee Nuntika, Punsawad Chuchard, Han Jaehong, Net-Anong Soiphet
Department of Applied Thai Traditional Medicine, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Research Center in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Antioxidants (Basel). 2023 Jul 15;12(7):1428. doi: 10.3390/antiox12071428.
Tri-Yannarose is a Thai traditional herbal medicine formula composed of , , and . It possesses antipyretic, diuretic, expectorant, and appetite-stimulating effects. This study aimed to evaluate the antioxidant activities, cytotoxicity, and chemical constituents of an aqueous extract following a Tri-Yannarose recipe and its plant ingredients. The phytochemical analysis was performed using LC-QTOF-MS. Antioxidant activities were determined using DPPH, ABTS, TPC, TFC, FRAP, NBT, MCA, and ORAC assays. Cytotoxicity was investigated using a methyl thiazol tetrazolium (MTT) assay. In addition, the relationship between the chemical composition of Tri-Yannarose and antioxidant activities was investigated by examining the structure-activity relationship (SAR). The results of the LC-QTOF-MS analysis revealed trigonelline, succinic acid, citric acid, and other chemical constituents. The aqueous extract of the recipe showed significant scavenging effects against ABTS and DPPH radicals, with IC values of 1054.843 ± 151.330 and 747.210 ± 44.173 µg/mL, respectively. The TPC of the recipe was 92.685 mg of gallic acid equivalent/g of extract and the TFC was 14.160 mg of catechin equivalent/g of extract. All extracts demonstrated lower toxicity in the Vero cell line according to the MTT assay. In addition, the SAR analysis indicated that prenyl arabinosyl-(1-6)-glucoside and quinic acid were the primary antioxidant compounds in the Tri-Yannarose extract. In conclusion, this study demonstrates that Tri-Yannarose and its plant ingredients have potent antioxidant activities with low toxicity. These results support the application of the Tri-Yannarose recipe for the management of a range of disorders related to oxidative stress.
三叶草玫瑰是一种泰国传统草药配方，由[此处原文缺失三种成分名称]组成。它具有解热、利尿、祛痰和刺激食欲的作用。本研究旨在评估按照三叶草玫瑰配方制备的水提取物及其植物成分的抗氧化活性、细胞毒性和化学成分。采用液相色谱-四极杆飞行时间质谱（LC-QTOF-MS）进行植物化学分析。使用DPPH、ABTS、总酚含量（TPC）、总黄酮含量（TFC）、铁还原抗氧化能力（FRAP）、氮蓝四唑（NBT）、亚油酸甲酯（MCA）和氧自由基吸收能力（ORAC）测定法测定抗氧化活性。使用甲基噻唑基四唑（MTT）测定法研究细胞毒性。此外，通过研究结构-活性关系（SAR）来探讨三叶草玫瑰的化学成分与抗氧化活性之间的关系。LC-QTOF-MS分析结果显示含有葫芦巴碱、琥珀酸、柠檬酸和其他化学成分。该配方的水提取物对ABTS和DPPH自由基具有显著的清除作用，IC值分别为1054.843±151.330和747.210±44.173µg/mL。该配方的总酚含量为92.685mg没食子酸当量/g提取物，总黄酮含量为14.160mg儿茶素当量/g提取物。根据MTT测定法，所有提取物在Vero细胞系中均表现出较低的毒性。此外，SAR分析表明异戊烯基阿拉伯糖基-(1-6)-葡萄糖苷和奎尼酸是三叶草玫瑰提取物中的主要抗氧化化合物。总之，本研究表明三叶草玫瑰及其植物成分具有强大的抗氧化活性且毒性较低。这些结果支持将三叶草玫瑰配方应用于治疗一系列与氧化应激相关的疾病。
