Rossi G L, Young D J, Wasserman S I, Barrett K E
Department of Medicine, University of California, San Diego School of Medicine.
Agents Actions. 1990 Nov;31(3-4):257-62. doi: 10.1007/BF01997617.
Changes in intracellular calcium levels induced in mast cells by either antigen or ionomycin were monitored using flow cytometry and the calcium binding dye indo-1. Both stimuli increased calcium levels in responsive cells by a similar amount, but not all cells examined were responsive to antigen. Antigen-induced increases in intracellular calcium levels could be completely blocked by the calcium antagonist TMB-8. Flow cytometry appears to be a useful method for monitoring mast cell calcium concentrations, as it provides the capability to study large numbers of individual cells.
使用流式细胞术和钙结合染料indo-1监测抗原或离子霉素诱导的肥大细胞内钙水平的变化。两种刺激均使反应性细胞中的钙水平升高相似的量，但并非所有检测的细胞都对抗原产生反应。钙拮抗剂TMB-8可完全阻断抗原诱导的细胞内钙水平升高。流式细胞术似乎是监测肥大细胞钙浓度的一种有用方法，因为它能够研究大量单个细胞。
