Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Poult Sci. 2010 Oct;89(10):2123-9. doi: 10.3382/ps.2010-00919.
Major histocompatibility complex has previously been shown to influence the resistance of chicken to Marek's disease virus (MDV). However, little is known about expression of other genes in the MHC-I and II pathway after MDV infection. This study aimed at investigating 8 immune-related genes in the MHC core region that affects host responses to MDV. Spleens of infected and age-matched uninfected chickens were removed at 4, 7, 14, 21, and 28 d postinfection for gene expression detection using real-time PCR. Different expression patterns of MHC-I and II pathway genes were observed in the spleen. In the MHC-I pathway, the expression of transporter of antigen protein 1 (TAP1), transporter of antigen protein 2 (TAP2), and transporter of antigen protein-binding protein (TAPBP) genes was significantly increased in the spleen of MDV-infected than that of uninfected chickens. It indicated that host antivirus responses were generated to enhance antigen presentation. However, MHC-II pathway genes showed contrary trends. Classical MHC-II β chain major gene (BLB2) and nonclassical class II genes [DM α chain gene (DMA), DM β chain gene-1 (DMB1), and DM β chain gene-2 (DMB2)] had consistent lower transcripts in spleens of MDV-infected than that of uninfected chickens, which reflected that MDV interfered with multiple components of the MHC-II pathway. Overall, expression of most genes in the MHC core region was altered; moreover, the genes in endogenous and exogenous antigen presentation pathways had different expression patterns in the spleen after MDV infection.
主要组织相容性复合体（MHC）先前已被证明会影响鸡对马立克氏病病毒（MDV）的抵抗力。然而，关于 MDV 感染后 MHC-I 和 II 途径中其他基因的表达知之甚少。本研究旨在研究影响宿主对 MDV 反应的 MHC 核心区域中的 8 个免疫相关基因。在感染后 4、7、14、21 和 28 天，从感染和年龄匹配的未感染鸡的脾脏中取出用于实时 PCR 基因表达检测。在脾脏中观察到 MHC-I 和 II 途径基因的不同表达模式。在 MHC-I 途径中，与未感染的鸡相比，在 MDV 感染的鸡的脾脏中，抗原蛋白转运体 1（TAP1）、抗原蛋白转运体 2（TAP2）和抗原蛋白结合蛋白转运体（TAPBP）基因的表达显着增加。这表明宿主产生了抗病毒反应以增强抗原呈递。然而，MHC-II 途径基因表现出相反的趋势。经典 MHC-II β 链主要基因（BLB2）和非经典类 II 基因[DM α 链基因（DMA）、DM β 链基因-1（DMB1）和 DM β 链基因-2（DMB2）]在 MDV 感染的鸡的脾脏中的转录本一致较低，这反映了 MDV 干扰了 MHC-II 途径的多个成分。总体而言，MHC 核心区域的大多数基因的表达发生了改变；此外，在 MDV 感染后，内源性和外源性抗原呈递途径的基因在脾脏中具有不同的表达模式。
