Guo Yujie, Su Aru, Tian Huihui, Zhai Minxi, Li Wenting, Tian Yadong, Li Kui, Sun Guirong, Jiang Ruirui, Han Ruili, Yan Fengbin, Kang Xiangtao
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, China.
Genes (Basel). 2020 May 6;11(5):513. doi: 10.3390/genes11050513.
Stress-induced immunosuppression is a common problem in the poultry industry, but the specific mechanism of its effect on the immune function of chicken has not been clarified. In this study, 7-day-old Gushi cocks were selected as subjects, and a stress-induced immunosuppression model was successfully established via daily injection of 2.0 mg/kg (body weight) dexamethasone. We characterized the spleen transcriptome in the control (B_S) and model (D_S) groups, and 515 significant differentially expressed genes (SDEGs) (Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced (FPKM) > 1, adjusted -value (padj) < 0.05 and Fold change (|FC|) ≥ 2) were identified. The cytokine-cytokine receptor interaction signaling pathway was identified as being highly activated during stress-induced immunosuppression, including the following SDEGs-, and . At the same time, immune-related SDEGs including , and were significantly enriched in the intestinal immune network for the IgA production signaling pathway. The SDEG protein-protein interaction module analysis showed that and may play an important role in stress-induced immunosuppression. These findings provide a background for further research on stress-induced immunosuppression. Thus, we can better understand the molecular genetic mechanism of chicken stress-induced immunosuppression.
应激诱导的免疫抑制是家禽业中一个常见问题，但它对鸡免疫功能影响的具体机制尚未阐明。在本研究中，选用7日龄固始公鸡作为研究对象，通过每日注射2.0 mg/kg（体重）地塞米松成功建立了应激诱导的免疫抑制模型。我们对对照组（B_S）和模型组（D_S）的脾脏转录组进行了表征，共鉴定出515个显著差异表达基因（SDEGs）（每百万碱基测序中每千碱基转录本序列的片段数（FPKM）>1，校正P值（padj）<0.05且折叠变化（|FC|）≥2）。细胞因子-细胞因子受体相互作用信号通路被确定在应激诱导的免疫抑制过程中高度激活，包括以下SDEGs——和。同时，包括、和在内的免疫相关SDEGs在肠道免疫网络中IgA产生信号通路中显著富集。SDEG蛋白质-蛋白质相互作用模块分析表明，和可能在应激诱导的免疫抑制中发挥重要作用。这些发现为进一步研究应激诱导的免疫抑制提供了背景。因此，我们可以更好地理解鸡应激诱导免疫抑制的分子遗传机制。
