Department of Anatomy, School of Medicine, Keimyung University, 2800 Dalgubeoldaero, Dalseo-Gu, Daegu, Republic of Korea.
Pathol Oncol Res. 2011 Jun;17(2):201-5. doi: 10.1007/s12253-010-9297-z. Epub 2010 Sep 18.
Uterine leiomyomas are benign tumors of the uterus that arise clonally from smooth muscle cells of the myometrium and are the most common reason for hysterectomies. The aim of this study was to evaluate mitochondrial microsatellite instability (mtMSI) in uterine leiomyomas and leiomyosarcomas to clarify the molecular pathogenetic distinction between these tumors. DNA was isolated from paired normal and tumoral tissues in 50 patients with uterine leiomyomas and 14 patients with leiomyosarcomas. mtMSI was analyzed by using eight microsatellite markers. Our result showed that mitochondrial microsatellite instability was not found in all uterine leiomyomas. However, 3 (21.4%) of 14 patients with leiomyosarcomas had mtMSI and the frequencies of mtMSI in these tumors were significantly different (p < 0.01). Distinctive characteristics of mitochondrial genetic instability in uterine leiomyomas and leiomyosarcomas suggested the potential of mtMSI as a marker for differential diagnosis between them.
子宫肌瘤是子宫的良性肿瘤，起源于子宫平滑肌细胞的克隆性，是子宫切除术最常见的原因。本研究旨在评估子宫肌瘤和平滑肌肉瘤中的线粒体微卫星不稳定性（mtMSI），以阐明这些肿瘤之间的分子发病机制区别。从 50 名子宫肌瘤患者和 14 名平滑肌肉瘤患者的配对正常和肿瘤组织中分离 DNA。使用 8 个微卫星标记物分析 mtMSI。我们的结果表明，并非所有子宫肌瘤均存在线粒体微卫星不稳定性。然而，14 名平滑肌肉瘤患者中有 3 名（21.4%）存在 mtMSI，这些肿瘤中 mtMSI 的频率有显著差异（p<0.01）。子宫肌瘤和平滑肌肉瘤中独特的线粒体遗传不稳定性特征表明 mtMSI 可能成为它们之间鉴别诊断的标志物。
