Reid G, Schwarz-Faulkner S, McGroarty J A, Bruce A W
Department of Surgery, University of Toronto, Ontario, Canada.
Perit Dial Int. 1990;10(1):21-4.
The ability of gram-positive cocci to aggregate and form biofilms at the exit site and on catheter surfaces in continuous ambulatory peritoneal dialysis (CAPD) has been associated with peritonitis. In the present study, 16 (80%) of 20 dialysate effluents from patients with staphylococcal peritonitis were found to contain aggregates of Staphylococcus epidermidis or S. aureus. Using an in vitro assay system, 20 (83%) of 24 pathogenic bacterial strains were found to aggregate in fresh dialysis solutions within 4 to 24 h at 37 degrees C. Further studies with one strain, S. epidermidis 1938, showed increased aggregation when the bacteria were incubated in dialysate effluents compared to fresh dialysis solutions. Viability experiments showed that aggregated bacteria were less sensitive to the killing effects of fresh dialysis solutions and that dialysate effluents could support bacterial growth. The results suggest that attention be paid to aggregation as a potential mechanism used by bacteria in causation of peritonitis.
在持续性非卧床腹膜透析（CAPD）中，革兰氏阳性球菌在出口部位和导管表面聚集并形成生物膜的能力与腹膜炎有关。在本研究中，20例葡萄球菌性腹膜炎患者的透析液流出物中有16例（80%）被发现含有表皮葡萄球菌或金黄色葡萄球菌聚集体。使用体外检测系统，24株致病细菌菌株中有20株（83%）在37℃下于新鲜透析液中4至24小时内聚集。对一株表皮葡萄球菌1938的进一步研究表明，与新鲜透析液相比，当细菌在透析液流出物中孵育时，聚集增加。活力实验表明，聚集的细菌对新鲜透析液的杀伤作用不太敏感，并且透析液流出物可以支持细菌生长。结果表明，应关注聚集作为细菌引起腹膜炎的潜在机制。
