Baddour L M, Smalley D L, Kraus A P, Lamoreaux W J, Christensen G D
Diagn Microbiol Infect Dis. 1986 Sep;5(3):197-205. doi: 10.1016/0732-8893(86)90002-7.
Twenty-one microbiologically documented episodes of coagulase-negative staphylococcal peritonitis occurred in 21 continuous ambulatory peritoneal dialysis patients. All strains involved in these infections were tested for antimicrobial susceptibility and in vitro adherence assays. Twenty of the strains were species identified using two commercially available systems. For comparison, 20 saprophytic strains of coagulase-negative staphylococci obtained from the nares and axillae of 10 uninfected, peritoneal dialysis patients were included for in vitro characterization. Staphylococcus epidermidis was the species most often identified for both clinical and saprophytic strains. Eighteen of the 21 (86%) clinical strains were resistant to penicillin G. Methicillin resistance, which was present in five clinical strains, was not found in saprophytic strains. Adherence assay determinations showed marked differences between clinical versus colonization strains, with the clinical isolates significantly more adherent (p less than 0.025) than colonization strains. Electron microscopic examination of silastic catheter segments incubated with a strain of S. epidermidis in used and unused dialysis fluids demonstrated marked differences in attachment of bacteria to catheter material.
21例持续非卧床腹膜透析患者发生了21次经微生物学证实的凝固酶阴性葡萄球菌腹膜炎。对这些感染中涉及的所有菌株进行了药敏试验和体外黏附试验。使用两种市售系统对其中20株菌株进行了菌种鉴定。作为对照，纳入了从10例未感染的腹膜透析患者的鼻腔和腋窝获得的20株腐生型凝固酶阴性葡萄球菌菌株进行体外特性分析。表皮葡萄球菌是临床菌株和腐生菌株中最常鉴定出的菌种。21株临床菌株中有18株（86%）对青霉素G耐药。5株临床菌株存在耐甲氧西林情况，而腐生菌株中未发现这种情况。黏附试验测定显示临床菌株与定植菌株之间存在显著差异，临床分离株的黏附性明显高于定植菌株（p<0.025）。对在使用过和未使用过的透析液中与一株表皮葡萄球菌一起孵育的硅橡胶导管段进行电子显微镜检查，结果表明细菌在导管材料上的附着存在显著差异。
