在克罗恩病中，有活力的分枝杆菌亚种副结核分枝杆菌的高流行率。
High prevalence of viable Mycobacterium avium subspecies paratuberculosis in Crohn's disease.
机构信息
Department of Gastroenterology, Hospital Clinico San Carlos, Martin Lagos s/n, 28040 Madrid, Spain.
出版信息
World J Gastroenterol. 2010 Sep 28;16(36):4558-63. doi: 10.3748/wjg.v16.i36.4558.
AIM
To examine the detection rate of viable Mycobacterium avium subspecies paratuberculosis (MAP) in patients with inflammatory bowel disease [Crohn's disease (CD) and ulcerative colitis (UC)].
METHODS
Thirty patients with CD (15 with at least one NOD2/CARD15 mutation), 29 with UC, and 10 with no inflammatory bowel disease (IBD). were tested for MAP by polymerase chain reaction (specific IS900 fragment) and blood culture.
RESULTS
MAP DNA was detected in all original blood samples and 8-wk blood cultures (CD, UC and non-IBD). Positive MAP DNA status was confirmed by dot blot assays. All 69 cultures were negative by acid-fast Ziehl-Neelsen staining. Viable MAP, in spheroplast form, was isolated from the 18-mo blood cultures of all 30 CD patients, one UC patient, and none of the non-IBD controls. No association was found between positive MAP cultures and use of immunosuppressive drugs or CD-associated single nucleotide polymorphisms.
CONCLUSION
MAP is widely present in our area and MAP DNA can be recovered from the blood of CD, UC and non-IBD patients. However, MAP spheroplasts were only found in CD patients.
目的
检测患有炎症性肠病（克罗恩病[CD]和溃疡性结肠炎[UC]）患者中可行的鸟分枝杆菌亚种副结核分枝杆菌（MAP）的检出率。
方法
对 30 名 CD 患者（15 名至少有一个 NOD2/CARD15 突变）、29 名 UC 患者和 10 名无炎症性肠病（IBD）患者进行聚合酶链反应（特异性 IS900 片段）和血培养检测 MAP。
结果
在所有原始血样和 8 周血培养（CD、UC 和非 IBD）中均检测到 MAP DNA。通过斑点印迹分析证实了 MAP DNA 的阳性状态。所有 69 个培养物均经抗酸 Ziehl-Neelsen 染色呈阴性。从 30 名 CD 患者、1 名 UC 患者和所有非 IBD 对照组的 18 个月血培养中分离出了可行的 MAP，呈原生质体形式。未发现 MAP 阳性培养物与免疫抑制剂的使用或与 CD 相关的单核苷酸多态性之间存在关联。
结论
MAP 在本地区广泛存在，MAP DNA 可从 CD、UC 和非 IBD 患者的血液中回收。然而，只有 CD 患者中发现了 MAP 原生质体。
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