[免疫印迹技术检测抗SM、RNP和SSR多肽抗体的临床意义]

[The clinical significance of antibodies to SM, RNP and SSR polypeptides detected by immunoblotting technique].

作者信息

Xu Y H, Chen S L, Ye P

机构信息

Ren Ji Hospital Affiliated to Shanghai Second Medical University.

出版信息

Zhonghua Nei Ke Za Zhi. 1990 Aug;29(8):472-5, 510.

PMID:2086016

Abstract

Using immunoblotting technique (IBT) antibodies to Sm, RNP and SSB polypeptides we detected in 173 patients with different rheumatic diseases. It was found that antibodies to Sm polypeptides with molecular weights of 28K(B) and 13.5K(D) could be detected almost only in SLE (34.0% and 30.0% respectively). The positive rates of anti-28K and anti-13.5K polypeptides in SLE detected by IBT were significantly higher than those of anti-Sm by counterimmunoelectrophoresis (12.0%) (P less than 0.05). The antibodies to SSB polypeptides with molecular weights of 48K and 43.41K could be detected mainly in Sjogren's syndrome (70.0% and 65.0% respectively) and the antibodies to RNP polypeptides with molecular weights of 68K, 32K(A) and 29K (B') mainly in MCTD (82.6% 100% and 34.8% respectively) (P less than 0.001). It was reported by Pettersson that anti-68K polypeptide had high specificity for the diagnosis of MCTD. But our data showed that the anti-68K polypeptide could also be detected in SLE(26.0%) and PSS(15.0%) and was not so specific for MCTD.

摘要

运用免疫印迹技术（IBT），我们检测了173例不同风湿性疾病患者体内抗Sm、RNP和SSB多肽的抗体。结果发现，分子量为28K（B）和13.5K（D）的抗Sm多肽抗体几乎仅在系统性红斑狼疮（SLE）患者中可检测到（分别为34.0%和30.0%）。通过免疫印迹技术检测的SLE患者中抗28K和抗13.5K多肽的阳性率显著高于对流免疫电泳检测的抗Sm阳性率（12.0%）（P<0.05）。分子量为48K和43.41K的抗SSB多肽抗体主要在干燥综合征患者中可检测到（分别为70.0%和65.0%），分子量为68K、32K（A）和29K（B'）的抗RNP多肽抗体主要在混合性结缔组织病（MCTD）患者中可检测到（分别为82.6%、100%和34.8%）（P<0.001）。Pettersson报道抗68K多肽对MCTD的诊断具有高特异性。但我们的数据显示，抗68K多肽在SLE（26.0%）和原发性干燥综合征（PSS，15.0%）患者中也可检测到，对MCTD并非如此特异。